- Research article
- Open Access
Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity
BMC Evolutionary Biology volume 20, Article number: 149 (2020)
Although the processes of co-evolution between parasites and their hosts are well known, evidence of co-speciation remains scarce. Microsporidian intracellular parasites, due to intimate relationships with their hosts and mixed mode of transmission (horizontal but also vertical, from mother to offspring), may represent an interesting biological model for investigating co-speciation. Amphipod crustaceans, especially gammarids, are regular hosts of microsporidian parasites, in particular the Dictyocoela spp., which have so far been found limited to these amphipods and are known to use a vertical mode of transmission. The amphipod genus Gammarus has a diversification history spanning the last 50–60 Mya and an extensive cryptic diversity in most of the nominal species. Here, we investigated the degree of co-diversification between Dictyocoela and Gammarus balcanicus, an amphipod with high degrees of ancient cryptic diversification and lineage endemism, by examining the genetic diversity of these parasites over the entire geographic range of the host. We hypothesised that the strong host diversification and vertical transmission of Dictyocoela would promote co-diversification.
Using the parasite SSU rDNA as a molecular marker, analyzing 2225 host specimens from 88 sites covering whole host range, we found 31 haplogroups of Dictyocoela, 30 of which were novel, belonging to four Dictyocoela species already known to infect other Gammarus spp. The relationships between Dictyocoela and gammarids is therefore ancient, with the speciation in parasites preceding those of the hosts. Each novel haplogroup was nevertheless specific to G. balcanicus, leaving the possibility for subsequent co-diversification process during host diversification. A Procrustean Approach to Co-phylogeny (PACo) analysis revealed that diversification of Dictyocoela was not random with respect to that of the host. We found high degrees of congruence between the diversification of G. balcanicus and that of Dictyocoela roeselum and D. muelleri. However, we also found some incongruences between host and Dictyocoela phylogenies, e.g. in D. duebenum, probably due to host shifts between different G. balcanicus cryptic lineages.
The evolutionary history of Dictyocoela and Gammarus balcanicus represents an example of an overall host-parasite co-diversification, including cases of host shifts.
The intimate relationships between symbionts and their hosts sometimes suggest that any event of speciation in the host lineage is likely to result in the simultaneous isolation event of species in its associated symbionts [1,2,3]. Such co-speciation patterns have been found mostly between mutualistic symbionts and their hosts , a phenomenon facilitated by some peculiar ecological conditions or life-history traits, such as by symbiont vertical transmission , but see . Cases of co-speciation between parasites and their hosts seems rarer . However, despite probable overestimates of this phenomenon , recent studies suggest its occurrence in various parasites taxa [2, 6, 7].
Because of their intimate relationships with their hosts, microsporidian parasites have been suggested as good candidates for showing some degrees of co-speciation, for example in Culex mosquitoes infected by Amblyospora microsporidia . Microsporidia are obligate unicellular endoparasites belonging to an extremely ancient and phylogenetically diverse phylum close to fungi . These ubiquitous parasites infect a wide range of vertebrate and invertebrate hosts . They are responsible for many diseases of insects and crustaceans [11,12,13]. Among aquatic arthropods, the freshwater amphipod crustaceans, especially those of the superfamily Gammaroidea, are commonly infected by microsporidia (for overviews see [12, 14, 15]). Nosema , Cucumispora  and Dictyocoela  commonly infect numerous gammarid species across Eurasia [18,19,20,21] and North America [22, 23].
In particular, Dictyocoela spp. form a monophyletic clade restricted to amphipod hosts. Many Dictyocoela species, or strains within species has been identified, mostly using molecular tools [18,19,20,21, 24,25,26,27,28]. However, a number of these variants were formally described as species using both molecular and morphological criteria [23, 24], namely Dictyocoela muelleri, D. roeselum, D. berillonum, D. duebenum and D. diporeiae. Most of them include some degrees of intra-taxa molecular divergence. These microsporidia infect a wide range of amphipod species, specifically Gammarus spp. [24, 29]. The life cycle of numerous Dictyocoela species is not known. In two hosts, Gammarus duebeni and G. roeselii, Dictyocoela roeselum, D. duebenum and D. muelleri are known to be vertically transmitted: they infect oocytes, and, therefore, are transmitted to most embryos [30,31,32]. These species induce low virulence  as well as sex-ratio distortion in their host populations. Indeed, the putative male host receiving the vertically transmitted parasites are reversed into functional females [34,35,36]. However, some strains of Dictyocoela duebenum are not sex ratio distorters , and almost all most of the strains of the species described by Bacela-Spychalska et al.  were also found to infect muscles. It indicates possible variation in life-history traits, such as transmission pathway or feminization, among Dictyocoela and/or according to the host species.
Most of the phylogenetic studies suggest little co-speciation between gammarids and the Dictyocoela species identified so far. Studies by  and  both showed that all these species of infect many host taxa. For example, Dictyocoela duebenum, discovered in the Northwestern European Gammarus duebeni, has been found infecting the Ponto-Caspian Dikerogammarus villosus, the Southwestern European Echinogammarus berilloni as well as the Baikal hosts Gmelinoides fasciatus and Brandtia latissima. Similarly, Dictyocoela muelleri infects the Ponto-Caspian gammarids D. villosus and Pontogammarus robustoides, plus Gammarus duebeni, G. roeselii and G. varsoviensis, the latter three species being from Northwestern, Southeastern and Central Europe, respectively . However, within each of these parasite species, a single Dictyocoela strain (based on the partial or total sequences of the SSU rDNA sequence) rarely share two host species. It, therefore, remains challenging to understand whether some Dictyocoela variants are specific for some hosts, or if Dictyocoela spp. are generalist parasites. The difficulty mainly comes from the fact that many of these studies were not based on the extensive sampling of hosts, but were based on rather punctual samples over large geographic areas or samples from limited geographic areas. For example,  showed that several species of hosts are infected by several microsporidian species/clades at a small geographic scale (a river drainage in the Ruhr region in Germany). Similarly,  showed that, in the peculiar context of the Lake Baikal gammarid radiation, microsporidia did not follow the differentiation of their hosts, but can instead be seen as generalist parasites exploiting different hosts. Moreover, host-parasite exchanges between Lake Baikal and surrounding rivers are frequent in this ecosystem. Conversely, at a larger scale, an extensive study of a single host (G. roeselii) over its whole geographic range suggests that there may be some degree of specificity within Dictyocoela roeselum, with some strains of this parasite species being restricted to infect G. roeselii only . However, other microsporidia species infecting this host result probably from host-shifts, from local amphipod fauna, following G. roeselii colonization of new geographic areas. More data of such kind are therefore needed to draw a more general picture of Dictyocoela-gammarid associations. A recent analysis of microsporidia in endemic New-Zealand amphipods indeed revealed an overall congruence between phylogenies of Paracalliope spp. and their Dictyocoela parasites. At a global scale, the observed pattern is similar between amphipods (beyond, but including, gammarids) and Dictyocoela. This pattern may have resulted from covicariance and/or codispersal, suggesting that the intimate association between amphipods and Dictyocoela may have persisted over macroevolutionary time .
Here, we add a third extensive census of Dictyocoela parasites in another gammarid host over its entire geographic range: Gammarus balcanicus, a morphospecies scarcely known for the presence of microsporidia. This host was chosen as a biological model for its high degree of diversification and divergence , making it particularly interesting to investigate parasite-host co-diversification. Gammarus balcanicus inhabits mountainous areas from the eastern Carpathians through the Balkan Peninsula, to the eastern Alps. However, some populations are also known from the Black Sea lowlands and in Crimea [39, 40]. The Carpathians and the Balkans are recognized as the most valuable present-day hot-spots of biodiversity and endemism, and a model system for studies upon biogeography and the evolution of numerous organisms [41, 42]. Notably, it is an ancient centre of diversity for some freshwater gammarids [39, 40, 43]. Gammarus balcanicus is characterized by high cryptic diversity, including at least 50 divergent MOTUs (Molecular Operational Taxonomic Units) of Miocene origin, clustered in seven main phylogenetic lineages (Fig. 1) . Within these lineages, the present-day G. balcanicus MOTUs are locally endemic, due to their complex phylogeographical history and habitat fragmentation. In a caricatural way, we could say that each small river basin harbours a separate MOTU of G. balcanicus. Indeed, the Balkan Peninsula and the Carpathian Arch have been characterized by landscape remodeling and high dynamic geographical complexity . Gammarus balcanicus species complex has started its diversification at ca. 20 Ma, in the early Miocene in the central Balkans, partially in the shallow epicontinental sea of Paratethys . This early diversification generated two major clades (Fig. 1): the AR clade, nowadays endemic to a small area in the Rhodope Mountains in the central Balkan Peninsula, and a huge clade which later (c. 15 Ma) split into the north-eastern clade (hereafter N) and the south-western clade (hereafter S). Subsequent diversification and geographic expansion of the north-eastern and south-western clades of G. balcanicus continued following the Alpine orogeny during Miocene/Pliocene and, finally, during the Pleistocene glaciations (Fig. 1) . Such a history makes this species complex a perfect model to test host-parasite co-diversification events.
The high degree of diversification and endemicity within G. balcanicus complex may provide ideal conditions for parasite co-diversification. This supposition is strengthened by the fact that Dictyocoela spp. found so far in this gammarid are the vertically-transmitted Dictyocoela muelleri [18, 29] and Dictyocoela roeselum , observed sporadically in Carpathian populations. The vertical-transmission of symbionts makes host-parasite co-diversification more probable . However, studies on microsporidian infections in G. balcanicus are too scarce to affirm that these parasites are the only ones infecting this species. Therefore, using PCR assays and DNA barcoding approach with the parasite SSU rDNA molecular marker, we investigated Dictyocoela spp. associated with G. balcanicus, in comparison with published data in other gammarids, to address the following issues: (1) Are there any Dictyocoela species or strains specific to G. balcanicus host? (2) Has the host phylogeographic history influenced host-parasite association and at what scale the co-diversifications can be observed between G. balcanicus and Dictyocoela parasites? Both the high degree of diversification and endemicity of the host and the vertical transmission of Dictyocoela should promote co-differentiation. This could occur at the scale of either between parasite species, if some Dictyocoela species infected G. balcanicus after its diversification, or between parasite strains within species, if associations between G. balcanicus and Dictyocoela is older than the host diversification. Owing to the results obtained on other gammarids at different geographic scales (see, e.g. [20, 24, 26]), we would predict the later hypothesis being more probable.
Sampling and total DNA extraction
Gammarus balcanicus individuals were gathered during several sampling campaigns between 2004 and 2016, at 88 sites in 13 countries, covering the entire distribution range of this morphospecies in Europe (Fig. 2, Additional file 1). The sites were plotted on a map using Qgis 2.18.4 (QGIS Development Team 2009). Samples were collected using hand nets and kick-sampling method. All individuals were immediately fixed in 96% ethanol at the sampling site and stored at room temperature after returning to the laboratory. Amphipods were identified to the morphospecies level using morphological characters described in available keys (e.g. [45, 46]). Samples used in the present study correspond to the G. balcanicus species complex samples used by . All the specimens are stored at the Department of Invertebrate Zoology and Hydrobiology, University of Lodz, Poland.
Each gammarid was dissected under a stereomicroscope. Approximately 2 mm3 of host tissue (including muscles and gonads) were taken from the 6th and 7th thoracic segments. Since microsporidia are intracellular parasites, their DNA was co-extracted with host DNA. Among the 2255 host individuals used in the present study, DNA was obtained from 1202 males and 1053 females (females were not available in all gathered samples). The DNA extraction was performed using either (i) standard phenol–chloroform protocol  or (ii) Biobasic EZ-10 96 Well Plate Genomic DNA Isolation Kit for Animal Sample and eluted in 100 µl of TE (pH 8). The DNA samples were kept at 4 °C until amplification and subsequently at − 20 °C for long-term storage.
Molecular screening for microsporidia
All the 2255 individuals were screened for the presence of microsporidia following the strategy described in , using the short (c. 350 bp long) diagnostic fragment of the small ribosomal subunit gene (SSU rDNA), amplified with the microsporidia-specific primers V1f (forward) (5′-CAC CAG GTT GAT TCT GCC TGA C-3′) paired with UNIr (reverse) (5′-TCA GGC TCC CTC TCC GGA AT-3′) . The use of this short fragment maximized the ability to detect the presence of microsporidians even in case of low infection intensity or partial degradation of DNA. As negative and positive controls in PCR reactions, we used, respectively, water and Dictyocoela roeselum DNA from Gammarus roeselii . The PCR conditions and visualization of PCR products were as described by .
In individuals positively diagnosed for microsporidian infections our objective, following , was to sequence the ca. 800 bp long fragment of the SSU rDNA gene matching the 5′ part either as one or two overlapping fragments. When the 800 bp long sequence was not obtainable, we used either a V1f-530r fragment (c. 530 bp long) or even a V1f-UNIr fragment (c. 350 bp long), that contained enough phylogenetic information to attribute sequences to the species level without any ambiguity (Additional file 2). PCR products were purified and sequenced directly with the BigDye technology by Genewiz, Inc., UK, using the forward primers from PCR. Using Geneious 10.2. . Raw sequences were edited and trimmed. Their microsporidian identity was confirmed using BlastN searches against sequences available in GenBank .
Phylogeny reconstruction for microsporidians and taxonomic assignment of newly produced sequences
For SSU rDNA, our dataset is composed of two types of microsporidian sequences (Additional file 2). First, newly produced sequences from the infected G. balcanicus individuals. Second, literature SSU sequences of Dictyocoela spp. found infecting European freshwater or brackish water amphipods, as in , with the addition of parasites from Lake Baikal [19, 26, 28], and from USA [23, 50, 51]. Sequences were aligned using MAFFT7.388 software , with the E-IONS-I algorithm using the legacy gap penalty option, incorporated in Geneious 10.2.2 . Our dataset contains sequences of different lengths both among the newly produced sequences and the published ones (Additional file 2). As some sequences were relatively short, reducing the full dataset to a standard size would, on the one hand, allow defining haplotypes but, on the other hand, would potentially induce losing the phylogenetic signal. Therefore, following the strategy described by , we attributed each sequence to a so-called haplogroup. Sequences belonging to distinct haplogroups harbored at least one or more variable sites, generating diagnostic features on the minimal length of a given haplogroup. Few sequences could not be assigned to only one haplogroup due to their short length and the resulting absence of diagnostic features. The longest sequence of each haplogroup was then used for the phylogeny reconstruction (Additional file 2). The best-fitting model of nucleotide substitution was determined with JModelTest-2.1.10  as being the General Time Reversible (GTR) model with gamma-distributed rate heterogeneity (G) and a significant proportion of invariable sites (I). Phylogenetic reconstructions were built using the Maximum Likelihood algorithm implemented in Mega-X  using extensive subtree-pruning-regrafting as ML heuristic method with very strong branch swap filtering and 1000 boostraps. Four sequences, AF044391 (Glugea anomala), GQ203287 (Glugea hertwigi), GQ246188 (Cucumispora dikerogammari) and KR190602 (Cucumispora ornata) were used as outgroup. Such a tree was aiming two purposes. First it presented an overall view of the diversity and divergence observed in the genus Dictyocoela. However, this tree did not aiming to provide a supported topology of clades at the genus level, given the very large taxa sampling, the use of a single marker and sequence being variable in length. Second, this tree allowed the taxonomical assignement of newly detected Dictyocoela sequences based on their phylogenetic proximity to published sequences, especially to sequences associated with formerly described taxa, i.e. D. muelleri, D. roeselum, D. berillonum and D. duebenum . In addition, a ML tree was built to provide support of the key clades presented in this paper. It was based on a subsample of individuals representative of diversity and divergence of all the Dictyocoela clades highlighted in the present paper, taking advantage of long SSU rDNA sequences, as well as ITS and LSU sequences when available. The tree was also based on GTR+G+I model, using 1000 bootstraps and the same outgroup. The topology of this tree is similar to the ones in  and .
Congruence between parasite and host phylogenies
The overall link between divergence patterns of Dictyocoela spp. and G. balcanicus was tested using Procrustean Approach to Co-phylogeny (PACo), one of the most conservative methods for analyzing co-phylogenies . PACo does not require fully resolved phylogenetic trees, which was the case for some parts of our parasite tree. This analysis can be based on genetic distances only. Therefore, we first constructed matrices of genetic distances for both the host and the parasites. For parasites, we used our newly produced sequences. For hosts, we used the COI (Cytochrome Oxidase I gene) haplotypes published by . As often as possible, we took the haplotype of the individual we found infected (Additional file 1). However, since we did not know the haplotypes of all our infected individuals, we took the host haplotypes representative of the BIN (Barcode Index Number) present in the population. The BIN is implemented as part of the Barcode of Life Data system (BOLD; . Sequences submitted to BOLD are clustered according to their molecular divergence and each cluster is ascribed a unique identifier (BIN), registered in BOLD. In our data set, there was one BIN per site, except for the HR05 site, where two BINs were present (Additional file 1). Since each BIN is a cluster of closely-related sequences, we took only one of these sequences as a representative of the BIN. For site # 50 (HR05), the two sequences were called 50 a and b. The matrices of genetic distances were computed with MEGA-X, using the Tamura-Nei substitution model (TN93, estimated by MEGA-X as being the best evolutionary model). These two distance matrices were linked by a binary matrix coding the host-parasite associations (see Fig. 5). Some parasites could be attributed to two haplogroups because of their short sequence length, resulting in losing some diagnostic sites (see above). We therefore constructed one binary matrix for each hypothesis. The ambiguous parasites were the following: For D. muelleri, matrices were build considering that individuals of the site # 56 (HR20) harbored a D. muelleri b07 haplogroup, or a b08 haplogroup. Similarly, gammarids from two sites (# 64, RO04 and # 74, RO36) harbored parasites of either D. roeselum b15 or b16 haplogroups. Different matrices were constructed to take this uncertainty into account (b15 in 64 – b16 in 74; b16- in 64 – b15 in 74; b15 in both sites; b16 in both sites). For each calculation, a residual sum of squares was then calculated as a global goodness-of-fit statistic between the host and parasite phylogenies (see  for details). Its significance was established by assigning hosts randomly to parasites on the parasite-host matrix with 1,000,000 permutations, testing the null hypothesis that there was no congruence between host and parasite phylogenies. The contribution of each host-parasite link to the global fit was assessed with a jackknife procedure that estimates the squared residual and its 95% confidence interval. The lower the residual, the higher its contribution to the global fit. For the sake of brevity and conservatism, in the main text and figures, we only presented the calculations for the hypothesis where the P values were the highest, all results being significant. However, the detailed results are provided in Additional file 3. The matrices used are also given in Additional file 3.
Dictyocoela species found in G. balcanicus and comparison with those of other hosts
In the 2255 analyzed individuals of G. balcanicus, overall, 31 haplogroups of Dictyocoela spp. were found infecting 139 individuals in 45 sites (Additional file 1). When placed onto the global phylogeny of Dictyocoela, the haplogroups infecting G. balcanicus could be ascribed to four fully described species of Dictyocoela parasites according to : Dictyocoela roeselum, D. muelleri, D. duebenum and D. berillonum (Additional files 4 and 5, Fig. 3). It is to be noticed that only one of these 31 haplogroups was shared with another gammarid host species (Droeb18, see later).
This global phylogeny confirms that few examples of specialization can be found in Dictyocoela spp: different host species or group of species shares most of these parasite species (Additional file 4). For example, the clade including Dictyocoela cavimanum and D. deshayesum is the only Dictyocoela clade infecting amphipods outside the infra-order Gammarida (sensu ) or gammaroids (sensu ) (Additional file 4). Dictyocoela of this clade infect Talitrida, an infra-order that diverged from gammaroids during early Mesozoic, ca. 180–200 MYA , and now distributed globally (e.g. Hyallela in America, Orchestia in Eurasia). However, parasites from this clade also infect a host species belonging to Baikal Acanthogammaridae (Ommatogammarus flavus) (Additional file 4). All other Dictyocoela infect only amphipods belonging to the Gammarida infra-order, but a single parasite species often infect host species from several gammarid subclades (as defined by ). For example, D. muelleri is found infecting species belonging to six clades: saline Gammarus group (Gammarus duebeni, G. aequicauda), G. roeselii group, G. lacustris group (G. varsoviensis), G. balcanicus group, G. pulex group, Ponto-Caspian group (sensu ) (Dikerogammarus villosus, D. haemobaphes, Pontogammarus robustoides) and three Baikal Acanthogammaridae (Eulimnogammarus vittatus, E. verrucosus and Acanthogammarus lappaceus) (Additional file 4). The host range of D. duebenum is even wider, including virtually all the host species groups of the Gammarida infra-order, but the Dinaric and Mediterranean Echinogammarus groups (Additional file 4). Despite its limited genetic diversity relative to other Dictyocoala, D. berillonum also has a wide host spectrum, with five host species groups infected (G. balcanicus, G. roeselii, pontogammarids, Marine and Atlantic Echinogammarus groups). Contrastingly, the host range of D. roeselum includes only Gammarus species from six different groups (G. lacustris, G. roeselii, G. balcanicus, G. pulex, G. fossarum and one saline Gammarus). Some of the Dictyocoela clades infecting Baikal amphipods seems restricted to this geographic area. Indeed, one Dictyocoela clade (Dictyocoela sp. Baikal 2) is limited to only one species of the oriental Gammarus group (Gmelinoides fasciatus) (Additional file 4). However, within this zone, some Dictyocoela species are also found in other geographic regions (D. muelleri and D. duebenum) (Additional file 4).
Diversity of Dictyocoela infecting the G. balcanicus morphospecies
The four species of Dictyocoela infecting G. balcanicus were not evenly represented when considering both haplogroup diversity and prevalence. Dictyocoela berillonum was found in seven individuals from four sites, only in the southern part of the G. balcanicus distribution (Fig. 4a, Additional files 1 and 2). While half of the 12 haplogroups of D. berillonum from the literature were found in two or more host species (Additional file 2, Fig. 3), the two haplogroups identified in the present study (Dberb01 and b02) were specific to G. balcanicus.
Dictyocoela duebenum was found in 36 individuals from 11 sites (Additional files 1 and 2) all over the range of G. balcanicus (Fig. 4a). Three new haplogroups were found, adding to those already known to be associated with other gammarid species, especially with G. duebeni [21, 35]. The three haplogroups infecting G. balcanicus were not shared with any of other host species (Fig. 3).
Dictyocoela muelleri was found in 48 individuals in 17 populations in Romania, Ukraine and Croatia, representing two geographically distinct zones, i.e. the N and S parts of G. balcanicus range (Fig. 4b, Additional files 1 and 2). Nine haplogroups were detected in our study, adding to the 29 haplogroups already detected in other gammarid species (Fig. 3, Additional file 2). These nine haplogroups can be grouped in two sets on the phylogenetic tree (Fig. 3). One set included haplogroups clustered within a single clade: Dmueb01, 02, 03, 05, 06, 07 and 08. The geographic distribution of these haplogroups was contrasting, with haplogroups Dmueb06, b07 and b08 restricted to the southern part of the distribution of G. balcanicus (Figs. 4b, 5) and haplogroups Dmueb01, b02, b03, b05 present only in the northern part of the host range and associated with the host clade #3 (Figs. 1, 4b, 5). The second set included haplogroups Dmueb10 and b11 found in four Romanian populations (Figs. 3, 4b). They were phylogenetically close to microsporidia infecting Gammarus roeselii, but also Ponto-Caspian Dikerogammarus haemobaphes and Pontogammarus robustoides (Fig. 3). The haplogroups Dmueb01 and b11 were found in hosts’ phylogenetic clades associated with both N and S geographic regions (Figs. 4b, 5).
Finally, Dictyocoela roeselum was the most widespread Dictycoela in G. balcanicus, detected in 46 individuals from 23 sites all over the host range (Figs. 3, 4c, Additional files 1 and 2). Dictyocoela roeselum was genetically diverse, accounting for 17 newly identified haplogroups. In addition to these new data, three other haplogroups were already reported for G. balcanicus by : MG773218, MG773221 and MG773220, and 17 other haplogroups were already known to infect other gammarid species. Again, each of these new haplogroups was specific to G. balcanicus, with the noticeable exception of Droeb18, identical to a haplogroup already found in G. roeselii  (Additional file 4, Fig. 3). Apart from this exception, others D. roeselum haplogroups were clustered into clades infecting only G. balcanicus (Fig. 3).
Co-diversification between Dictyocoela and G. balcanicus?
The PACo analysis revealed that the association between evolutionary divergences of G. balcanicus and Dictyocoela spp. was not random (P = 0.00003, see Fig. 6a). The individual links with low values of squared residuals, i.e. those contributing the most to the co-phylogeny, were found mostly in D. muelleri and D. roeselum (see below) (Fig. 6a). However, the squared residuals in links involving the site #26, i.e. the only relict population of the AR host clade (Fig. 1), were high, meaning that they did not contributed to the overall congruence of phylogenies. Similarly, most of the individual links involving D. duebenum contributed little to the overall congruence (Fig. 6a).
At the specific level in Dictyocoela (at least the species where haplogroups were numerous enough to run an analysis, i.e. D. muelleri and D. roeselum), associations between host and parasite phylogenies were not random either. It was the case for D. muelleri (P = 0.00047, Fig. 6b). Indeed, two groups of host sites shared closely-related D. muelleri haplogroups. The first group consisted of localities #62, 69, 77, and 78 located in the N part of the host distribution and phylogeny, the second group consisted of sites #50, 51, 52, 53 and 55, located in the S part of the host distribution/phylogeny (Figs. 2, 5). These two groups contributed importantly to the observed high probability of co-phylogeny, as revealed by their relatively low values of squared residuals (Fig. 6b). Similarly, a strong signal of co-phylogeny was also found between D. roeselum and G. balcanicus (P = 0.00406, Fig. 6c). The cluster of haplogroups Droeb11 - b12 - b13 was infecting hosts from sites in the northern part of the G. balcanicus range, mostly the host phylogenetic clade #3 (Fig. 5). These host-parasite couples generally contributed strongly to the general phylogenetic link (low square residual values, Fig. 6c). A similar high contribution to the host-parasite links was found for the parasite cluster grouping haplogroups Droeb02 b03, b04, b05, b06, b07 and b14 that infected hosts from the southern part of the geographical distribution (Figs. 4c, 5, 6c). Haplogroups Droeb09 and b10 were found only in the Crimean Peninsula (Fig. 4c). They were associated only with the host clade #1 (Figs. 1, 5) and also contributed highly to the host-parasite link (Fig. 6c). Conversely, the haplogroups Droeb01 and b08, while restricted to the southern parts of G. balcanicus range, were present in distantly-related host phylogenetic clades and contributed relatively little to the general host-parasite link (Figs. 5, 6c). Droeb08 also infected hosts from the population 26, which, as noted for the analysis involving all Dictyocoela species, therefore contributed little to the overall congruence (Fig. 6c). Similarly, Droeb18, associated with only one individual found in Hungary (Fig. 4c), showed a relatively high value of the squared residual (Fig. 6c). This haplogroup was 100% identical to Dictyocoela infecting G. roeselii (Genbank: AY584252, [24, 31]). It is worth noting that G. roeselii is sympatric with G. balcanicus in site #37, where this infected individual was found .
The exploration of Dictyocoela infections in Gammarus balcanicus adds 31 haplogroups to this genus of microsporidia. The phylogenetic reconstruction and inclusion to the general phylogeny built by  and , to which we added recently-published sequences from the Baikal region [19, 26, 28], allowed us to assign these genotypes unambiguously to four already-identified species: D. muelleri, D. roeselum, D. duebenum and D. berillonum. No new Dictyocoela highly divergent major clade was found in our samples of Gammarus balcanicus. However, our new sequences offered new insights for the analysis of a fascinating Russian-stacking-doll pattern of specificity.
At a global level, our expanded dataset confirmed that no strict specificity could be found between Dictyocoela species and their hosts. Most species or major clades of Dictyocoela were found infecting several host species, some of them being distantly phylogenetically related within amphipods . Few degrees of specialization can be found. The only example is the Dictyocoela clade infecting the Baikal amphipod species Gmelinoides fasciatus. Some other Baikal Dictyocoela infect several gammarid species in this geographic area. In addition, Gammarus lacustris, which is present in Lake Baikal system but has a much wider geographic distribution , can be infected by both typical Baikal Dictyocoela or other Dictyocoela strains from other regions (see Fig. 3, see also  for an example). Therefore, some Dictyocoela strains can be considered as endemic to Lake Baikal system, rather than host-specific, probably after a process of local selection or genetic drift. Conversely, however, Dictyocoela species found elsewhere (D. muelleri and D. duebenum) can infect amphipods from the Baikal group (Fig. 3), confirming the hypothesis of  that Dictyocoela are circulating among hosts within this geographic area. All other Dictyocoela clades/species infected species belonging to Gammarida, but a single parasite species often infect host species belonging to different clades . In addition, several hosts often share a single genotype of the parasite (as estimated by its SSU rDNA sequence), but this rule suffers a number of exceptions (e.g. in D. roeselum). In their co phylogenetic analysis, Park et al.  showed that there is reasonable evidence for a global Dictyocoela-amphipod codivergence, despite a general lack of specificity. Altogether, these results suggest that infection by Dictyocoela in amphipods is very ancient (see also ). The common ancestor should be at least older than the divergence between Talitrida and Gammarida, i.e. during early Mesozoic, ca. 180–200 MYA . The major diversification of Dictyocoela should have taken place before host diversification because most parasite groups infect diverse host groups . The only exception could be D. roeselum that infect only hosts of the Gammarus genus. This parasite species could therefore have emerged at the same times as the Gammarus groups differentiated from other amphipods, i.e. around 50–60 MYA according to .
Going down to the level of a single host species (or more precisely to a group of cryptic species, see ; ), such as Gammarus balcanicus studied here, confirms that it can be infected by several Dictyocoela species. However, our PACo analyses revealed that diversification of the parasites was not random with respect to host diversification, both between Dictyocoela species and at the level of diversification within each parasite species. The degree of host-parasite co-phylogenetic congruence was high between Dictyocoela species and G. balcanicus cryptic diversity shown by . As noted by , methods testing co phylogenies tend to overestimate real host-parasite co-speciations and any observation of congruence between two phylogenetic patterns should be examined with caution. In the case of G. balcanicus, we observed some striking patterns of such congruence, notably within D. roeselum and D. muelleri. We, therefore, think that co-differentiation between strains within these two Dictyocoela species and G. balcanicus clades could drive the overall Dictyocoela-G. balcanicus pattern. Indeed, within the two aforementioned parasite species, the divergence between main clades match well the early divergence of the northern and southern (i.e. N and S) host clades. In D. roeselum, two parasite groups seem to have split following this pattern (Figs. 4, 5, 6c) and then diversified within the two host lineages and biogeographical regions. Diversification occurred even within populations, as observed in sites ALP09 in Italy (#15) and HR21 in Croatia (#57) (Fig. 4, Additional file 1). The high degree of endemism and local differentiation of the different of G. balcanicus (Mamos et al. 2016) probably allowed some local adaptation and red-queen phenomenon of selection, leading to local diversification in parasites . A similar type of pattern was found for D. muelleri haplogroups Dmueb07 and 08, which were found only in the southern geographical region of G. balcanicus, linked only with the host clade #7 that differentiated there (Figs. 1, 5). A hypothesis alternative to co-differentiation could be that Dictyocoela diversity follows a geographical pattern independently of that of G. balcanicus, for example following the pattern of a putative intermediate or reservoir hosts, not detected yet because of sample bias. While we cannot exclude that Dictyocoela may infect other aquatic organisms (such a hypothesis is worth examining), it seems improbable to us. The only available survey—to our best knowledge—made on 12 macroinvertebrate host taxa in the same river indicates that, while numerous freshwater invertebrates may share some species-level-taxa microsporidian parasite in the same stream, Dictyocoela spp. were not found in taxa other than gammarids . Also, even if relatively scarce, investigations in aquatic organisms other than amphipods or fish led to the discovery of several new microsporidian species, but not Dictyocoela (e.g. for the most recent [63,64,65,66,67,68,69,70]), contrasting with the studies made on amphipods of different clades where Dictyocoela were easily found (e.g. for the most recent [19, 26,27,28, 38]. The closest relative of Dictyocoela spp. is Diplokaryon legeri (Figure S2 in ), a microsporidium infecting digenean parasite of marine bivalves [65, 71]. Even if hyperparasitism (i.e. parasitic organism infecting a parasite) would be and efficient pathway for host-shifts , such a hypothesis is not supported by the current data, Unikaryon being too far from Dictyocoela phylogenetically and ecologically.
Nevertheless, we found some obvious incongruences between the host and Dictyocoela phylogenies. One example is Dictyocoela duebenum, for which all detected haplogroups share host genotypes belonging to very different clades, and where most of the individual host-parasite links contributed poorly to the general host-parasite phylogenetic congruence (Fig. 6). Dictyocoela duebenum infect a wide range of hosts, where one single haplogroup may be shared between several host species (Additional file 4; ). This parasite could therefore be a generalist, characterized by high rate of host shifts. Another noticeable incongruence is found between the two parasites infecting gammarids from the site #26 (Fig. 6a, c), i.e. the relict AR lineage of the most ancient host vicariance (Fig. 1). These parasites are also found to infect individuals belonging to other host clades, from sites located in the southern geographic range of G. balcanicus, where site #26 is also located (Figs. 2, 5). This population may, therefore, have been infected by parasites transferred from neighboring sites rather than by parasites having a common phylogenetic history.
De Vienne et al.  showed that convincing examples of co-speciation between hosts and parasites seem to be rather an exception than a rule. They suggested that the methods testing co-phylogenies overestimates the phylogenetic links, and they evidenced that, in most cases, the detected congruence corresponded in fact to host shifts followed by specialization instead of co-speciation events. However, even if host shifts are obvious in our dataset, we do not believe that this explains the general pattern of our Dictyocoela—G. balcanicus phylogenetic congruence. First, as stated earlier, the divergence between the two main groups of parasites match well the early divergence of the hosts (, Figs. 1, 4). Second, the intimate link between microsporidia, intracellular parasites, and their hosts, as well as the vertical mode of transmission found in D. muelleri and D. roeselum (e.g. ), may favor co-speciation. It was already evidenced between hosts and mutualistic, vertically-transmitted, symbionts . Third, a co-vicariance has already been suggested for explaining the congruent phylogenies between New-Zealand Dictyocoela spp. and their Paracalliope spp. hosts . Our results would therefore confirm the pattern observed in New-Zealand fauna. However, since most microsporidia use mixed transmission strategy [12, 34] and because we also evidenced some host shifts in our data, we believe that the term ‘co-speciation’ is too firm to apply to our case study, and we suggest to use ‘co-diversification’. Patterns of co-diversification with diverse degrees of host shifts has been suggested for other host-microsporidia systems, notably Siberian mosquitoes and their microsporidia , and Gammarus roeselii and Nosema granulosis . Microsporidia may therefore be a parasite clade where finding co-diversification would be frequent. Indeed, while such a pattern has been found in some other host-parasite pairs (e.g. [6, 7]), it remains overall quite rare .
Based on all our data, we propose the following scenario for Dictyocoela diversification in G. balcanicus. Dictyocoela parasites, already diversified at the species level, infected common ancestors of G. balcanicus, before 15 MYA, the date around which differentiation of G. balcanicus began . Then, host-parasite co-diversification could have potentially occurred for each parasite species, following the diversification of the host (Fig. 1). The most apparent vicariance occurred early during the host diversification, separating the two host clades (N and S) inhabiting the northern and southern regions, profoundly impacting host-parasite association. For D. roeselum and D. muelleri, the host-parasite co-differentiation continued within each region, sometimes at very local scales, after this first major event. However, a number of subsequent horizontal transfers also occurred in the meantime (e.g. D. roeselum haplogroups b01 or b08, D. muelleri haplogroup b10). At least one parasite species, D. duebenum, did not show a strong pattern of co-diversification, and could have kept the potential to infect all host groups. This is perhaps due to its important capacity for host shifts, as proposed by . Therefore, our results point out that the relationships between microsporidia (particularly Dictyocoela) and amphipods (particularly Gammarida) could make a good biological model to investigate host-parasite co-diversification. It would be interesting to investigate in-depth some other target species (or group of closely-related species) of amphipod hosts, to understand the relative rates of co diversification vs. host shifts. Pan-European species like Gammarus pulex or G. fossarum could be excellent models as they are geographically widespread [72, 73] and already known to be infected by microsporidia in some parts of their range [20, 26].
Availability of data and materials
Material is available at the Department of Invertebrate Zoology and Hydrobiology, University of Lodz, Poland, upon request. GenBank accession numbers of partial SSU rDNA sequences generated in this study are MT932330-MT932461.
Barcode Index Number
Barcode of Life Data system
Cytochrome oxydase I gene
Million years ago
Molecular operational taxonomic unit
- SSU rDNA:
Small subunit ribosomal gene
Procrustean Approach to Co-phylogeny
Page RDM. Temporal congruence revisited: comparison of mitochondrial DNA sequence divergence in cospeciating pocket gophers and their chewing lice. Syst Biol. 1996;45:151–67.
Page RDM. Tangled trees: phylogeny, cospeciation, and coevolution. Chicago: University of Chicago Press; 2003.
de Vienne DM, Refrégier G, López-Villavicencio M, Tellier A, Hood ME, Giraud T. Cospeciation vs host-shift speciation: methods for testing, evidence from natural associations and relation to coevolution. New Phytol. 2013;198:347–85.
Jousselin E, Desdevises Y, Coeur d’acier A. Fine-scale cospeciation between Brachycaudus and Buchnera aphidicola: bacterial genome helps define species and evolutionary relationships in aphids. Proc R Soc B. 2009;276:187–96.
DeWayne SD, Machado CA, Molbo D, Werren JH, Windsor DM, Herre EA. The distribution of Wolbachia in fig wasps: correlations with host phylogeny, ecology and population structure. Proc R Soc Lond B. 2002;269:2257–67.
Megía-Palma R, Martínez J, Cuervo JJ, Belliure J, Jiménez-Robles O, Gomes V, et al. Molecular evidence for host–parasite co-speciation between lizards and Schellackia parasites. Int J Parasitol. 2018;48:709–18.
Pérez SD, Grummer JA, Fernandes-Santos RC, José CT, Medici EP, Marcili A. Phylogenetics, patterns of genetic variation and population dynamics of Trypanosoma terrestris support both coevolution and ecological host-fitting as processes driving trypanosome evolution. Parasit Vectors. 2019;12:473.
Andreadis TG, Simakova AV, Vossbrinck CR, Shepard JJ, Yurchenko YA. Ultrastructural characterization and comparative phylogenetic analysis of new microsporidia from Siberian mosquitoes: evidence for coevolution and host switching. J Invertebr Pathol. 2012;109:59–75.
Capella-Gutiérrez S, Marcet-Houben M, Gabaldón T. Phylogenomics supports microsporidia as the earliest diverging clade of sequenced fungi. BMC Biol. 2012;10:47.
Keeling PJ, Fast NM. Microsporidia: biology and evolution of highly reduced intracellular parasites. Annu Rev Microbiol. 2002;56:93–116.
Becnel JJ, Andreadis TG. Microsporidia in insects. In: Microsporidia. Hoboken: Wiley; 2014. p. 521–70. https://0-doi-org.brum.beds.ac.uk/10.1002/9781118395264.ch21.
Stentiford GD, Feist SW, Stone DM, Bateman KS, Dunn AM. Microsporidia: diverse, dynamic, and emergent pathogens in aquatic systems. Trends Parasitol. 2013;29:567–78.
Stentiford GD, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams B, et al. Microsporidia—emergent pathogens in the global food chain. Trends Parasitol. 2016;32:336–48.
Grabner DS. Hidden diversity: parasites of stream arthropods. Freshw Biol. 2017;62:52–64.
Weiss LM, Becnel JJ. Microsporidia: pathogens of opportunity. Hoboken: Wiley; 2014.
Naegeli K. Über die neue Krankheit der Seidenraupe und verwandte Organismen. Bot Ztg. 1857;15:760–1.
Ovcharenko MO, Bacela K, Wilkinson T, Ironside JE, Rigaud T, Wattier RA. Cucumispora dikerogammari n. gen. (Fungi: Microsporidia) infecting the invasive amphipod Dikerogammarus villosus: a potential emerging disease in European rivers. Parasitology. 2010;137:191–204.
Terry RS, Smith JE, Sharpe RG, Rigaud T, Littlewood DTJ, Ironside JE, et al. Widespread vertical transmission and associated host sex–ratio distortion within the eukaryotic phylum Microspora. Proc R Soc Lond B. 2004;271:1783–9.
Dimova M, Madyarova E, Gurkov A, Drozdova P, Lubyaga Y, Kondrateva E, et al. Genetic diversity of Microsporidia in the circulatory system of endemic amphipods from different locations and depths of ancient Lake Baikal. PeerJ. 2018;6:e5329.
Grabner DS, Weigand AM, Leese F, Winking C, Hering D, Tollrian R, et al. Invaders, natives and their enemies: distribution patterns of amphipods and their microsporidian parasites in the Ruhr Metropolis, Germany. Parasit Vectors. 2015;8:419.
Krebes L, Blank M, Frankowski J, Bastrop R. Molecular characterisation of the Microsporidia of the amphipod Gammarus duebeni across its natural range revealed hidden diversity, wide-ranging prevalence and potential for co-evolution. Infect Genet Evol. 2010;10:1027–38.
Galbreath Slothouber JGM, Terry RS, Becnel JJ, Dunn AM. Invasion success of Fibrillanosema crangonycis, n.sp., n.g.: a novel vertically transmitted microsporidian parasite from the invasive amphipod host Crangonyx pseudogracilis. Int J Parasitol. 2004;34:235–44.
Winters AD, Faisal M. Molecular and ultrastructural characterization of Dictyocoela diporeiae n. sp. (Microsporidia), a parasite of Diporeia spp. (Amphipoda, Gammaridea). Parasite. 2014;21:26.
Bacela-Spychalska K, Wróblewski P, Mamos T, Grabowski M, Rigaud T, Wattier R, et al. Europe-wide reassessment of Dictyocoela (Microsporidia) infecting native and invasive amphipods (Crustacea): molecular versus ultrastructural traits. Sci Rep. 2018;8:8945.
Bojko J, Dunn AM, Stebbing PD, Ross SH, Kerr RC, Stentiford GD. Cucumispora ornata n. sp. (Fungi: Microsporidia) infecting invasive ‘demon shrimp’ (Dikerogammarus haemobaphes) in the United Kingdom. J Invertebr Pathol. 2015;128:22–30.
Ironside JE, Wilkinson TJ. Accumulation and exchange of parasites during adaptive radiation in an ancient lake. Int J Parasitol. 2018;48:297–307.
Quiles A, Bacela-Spychalska K, Teixeira M, Lambin N, Grabowski M, Rigaud T, et al. Microsporidian infections in the species complex Gammarus roeselii (Amphipoda) over its geographical range: evidence for both host–parasite co-diversification and recent host shifts. Parasit Vectors. 2019;12:327.
Drozdova P, Madyarova E, Dimova M, Gurkov A, Vereshchagina K, Adelshin R, et al. The diversity of microsporidian parasites infecting the Holarctic amphipod Gammarus lacustris from the Baikal region is dominated by the genus Dictyocoela. J Invertebr Pathol. 2020;170:107330.
Bojko J, Ovcharenko M. Pathogens and other symbionts of the Amphipoda: taxonomic diversity and pathological significance. Dis Aquat Organ. 2019;136:3–36.
Dubuffet A, Smith JE, Solter L, Perotti MA, Braig HR, Dunn AM. Specific detection and localization of microsporidian parasites in invertebrate hosts by using in situ hybridization. Appl Environ Microbiol. 2013;79:385–8.
Haine ER, Brondani E, Hume KD, Perrot-Minnot M-J, Gaillard M, Rigaud T. Coexistence of three microsporidia parasites in populations of the freshwater amphipod Gammarus roeseli: evidence for vertical transmission and positive effect on reproduction. Int J Parasitol. 2004;34:1137–46.
Terry RS, Smith JE, Bouchon D, Rigaud T, Duncanson P, Sharpe RG, et al. Ultrastructural characterisation and molecular taxonomic identification of Nosema granulosis n. sp., a transovarially transmitted feminising (TTF) microsporidium. J Eukaryot Microbiol. 1999;46:492–9.
Gismondi E, Rigaud T, Beisel J-N, Cossu-Leguille C. Microsporidia parasites disrupt the responses to cadmium exposure in a gammarid. Environ Pollut. 2012;160:17–23.
Dunn AM, Smith JE. Microsporidian life cycles and diversity: the relationship between virulence and transmission. Microbes Infect. 2001;3:381–8.
Ironside JE, Alexander J. Microsporidian parasites feminise hosts without paramyxean co-infection: support for convergent evolution of parasitic feminisation. Int J Parasitol. 2015;45:427–33.
Jahnke M, Smith JE, Dubuffet A, Dunn AM. Effects of feminizing microsporidia on the masculinizing function of the androgenic gland in Gammarus duebeni. J Invertebr Pathol. 2013;112:146–51.
Wilkinson TJ, Rock J, Whiteley NM, Ovcharenko MO, Ironside JE. Genetic diversity of the feminising microsporidian parasite Dictyocoela: new insights into host-specificity, sex and phylogeography. Int J Parasitol. 2011;41:959–66.
Park E, Jorge F, Poulin R. Shared geographic histories and dispersal contribute to congruent phylogenies between amphipods and their microsporidian parasites at regional and global scales. Mol Ecol. 2020. https://0-doi-org.brum.beds.ac.uk/10.1111/mec.15562.
Mamos T, Wattier R, Burzyński A, Grabowski M. The legacy of a vanished sea: a high level of diversification within a European freshwater amphipod species complex driven by 15 My of Paratethys regression. Mol Ecol. 2016;25:795–810.
Copilaş-Ciocianu D, Petrusek A. Phylogeography of a freshwater crustacean species complex reflects a long-gone archipelago. J Biogeogr. 2017;44:421–32.
Médail F, Diadema K. Glacial refugia influence plant diversity patterns in the Mediterranean Basin. J Biogeogr. 2009;36:1333–45.
Poulakakis N, Kapli P, Lymberakis P, Trichas A, Vardinoyiannis K, Sfenthourakis S, et al. A review of phylogeographic analyses of animal taxa from the Aegean and surrounding regions. J Zool Syst Evol Res. 2015;53:18–32.
Grabowski M, Mamos T, Bącela-Spychalska K, Rewicz T, Wattier RA. Neogene paleogeography provides context for understanding the origin and spatial distribution of cryptic diversity in a widespread Balkan freshwater amphipod. PeerJ. 2017;5:e3016.
Popov SV, Rögl F, Rozanov AY, Steininger FF, Shcherba IG, Kovac M. Lithological-paleogeographic maps of Paratethys—10 maps late Eocene to Pliocene. 2004. https://www.schweizerbart.de/publications/detail/isbn/9783510613700/Lithological_Paleogeographic_maps_of_Paratethys_10. Accessed 14 Apr 2020.
Karaman GS, Pinkster S. Freshwater Gammarus species from Europe, north Africa and adjacent regions of Asia (Crustacea-Amphipoda). Part I. Gammarus pulex-group and related species. Bijdr TOT Dierkd. 1977a;47:1–86.
Karaman GS, Pinkster S. Freshwater Gammarus species from Europe, north Africa and adjacent regions of Asia (Crustacea-Amphipoda). Part II. Gammarus roeseli-group and related species. Bijdr TOT Dierkd. 1977b;47:165–96.
Hillis DM, Moritz C, Mable BK. Molecular systematics. 2nd ed. Sunderland, MA: Sinauer Associates, Inc.; 1996.
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, et al. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 2012;28:1647–9.
Madden T. The BLAST sequence analysis tool—the NCBI handbook—NCBI bookshelf. 2003. https://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/books/NBK153387/. Accessed 14 Apr 2020.
Ryan JA, Kohler SL. Virulence is context-dependent in a vertically transmitted aquatic host–microparasite system. Int J Parasitol. 2010;40:1665–73.
Slothouber Galbreath JGM, Smith JE, Becnel JJ, Butlin RK, Dunn AM. Reduction in post-invasion genetic diversity in Crangonyx pseudogracilis (Amphipoda: Crustacea): a genetic bottleneck or the work of hitchhiking vertically transmitted microparasites? Biol Invasions. 2010;12:191–209.
Katoh K, Standley DM. MAFFT Multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30:772–80.
Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods. 2012;9:772–772.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35:1547–9.
Balbuena JA, Míguez-Lozano R, Blasco-Costa I. PACo: a novel procrustes application to cophylogenetic analysis. PLoS ONE. 2013;8:e61048.
Ratnasingham S, Hebert PDN. bold: the barcode of life data system (https://www.barcodinglife.org). Mol Ecol Notes. 2007;7:355–64.
Lowry JK, Myers AA. A phylogeny and classification of the Amphipoda with the establishment of the new order Ingolfiellida (Crustacea: Peracarida). Zootaxa. 2017;4265:1–89.
Copilaş-Ciocianu D, Borko Š, Fišer C. The late blooming amphipods: global change promoted post-Jurassic ecological radiation despite Palaeozoic origin. Mol Phylogenet Evol. 2020;143:106664.
Hou Z, Sket B, Li S. Phylogenetic analyses of Gammaridae crustacean reveal different diversification patterns among sister lineages in the Tethyan region. Cladistics. 2014;30:352–65.
Hou Z, Sket B. A review of Gammaridae (Crustacea: Amphipoda): the family extent, its evolutionary history, and taxonomic redefinition of genera: phylogenetics of Gammaridae. Zool J Linn Soc. 2016;176:323–48.
Alther R, Fišer C, Altermatt F. Description of a widely distributed but overlooked amphipod species in the European Alps. Zool J Linn Soc. 2017;179:751–66.
Woolhouse MEJ, Webster JP, Domingo E, Charlesworth B, Levin BR. Biological and biomedical implications of the co-evolution of pathogens and their hosts. Nat Genet. 2002;32:569–77.
Sokolova Y, Pelin A, Hawke J, Corradi N. Morphology and phylogeny of Agmasoma penaei (Microsporidia) from the type host, Litopenaeus setiferus, and the type locality, Louisiana, USA. Int J Parasitol. 2015;45:1–16.
Sokolova YY, Senderskiy IV, Tokarev YS. Microsporidia Alfvenia sibirica sp. n. and Agglomerata cladocera (Pfeiffer) 1895, from Siberian microcrustaceans and phylogenetic relationships within the “Aquatic outgroup” lineage of fresh water microsporidia. J Invertebr Pathol. 2016;2016(136):81–91.
Stentiford GD, Ramilo A, Abollo E, Kerr R, Bateman KS, Feist SW, et al. Hyperspora aquatica n.gn., n.sp. (Microsporidia), hyperparasitic in Marteilia cochillia (Paramyxida), is closely related to crustacean-infecting microspordian taxa. Parasitology. 2017;144:186–99.
Costa G, Melo-Moreira E, de Carvalho MAP. Occurrence of microsporidians Glugea hertwigi and Pleistophora ladogensis, in smelt Osmerus eperlanus from two German rivers, North Sea coast. Dis Aquat Organ. 2016;121:49–57.
Sokolova YY, Overstreet RM. A new microsporidium, Apotaspora heleios n. g., n. sp., from the Riverine grass shrimp Palaemonetes paludosus (Decapoda: Caridea: Palaemonidae). J Invertebr Pathol. 2018;157:125–35.
Bojko J, Behringer DC, Moler P, Reisinger L. Ovipleistophora diplostomuri, a parasite of fish and their trematodes, also infects the crayfish Procambarus bivittatus. J Invertebr Pathol. 2020;169:107306.
Stentiford GD, Ross S, Minardi D, Feist SW, Bateman KS, Gainey PA, et al. Evidence for trophic transfer of Inodosporus octospora and Ovipleistophora arlo n. sp. (Microsporidia) between crustacean and fish hosts. Parasitology. 2018;145:1105–17.
Bojko J, Behringer DC, Moler P, Stratton CEL, Reisinger L. A new lineage of crayfish-infecting Microsporidia: the Cambaraspora floridanus n. gen. n. sp. (Glugeida: Glugeidae) complex from Floridian freshwaters (USA). J Invertebr Pathol. 2020;171:107345.
Canning EU, Nicholas JP. Light and electron microscope observations on Unikaryon legeri (Microsporida, Nosematidae), a parasite of the metacercaria of Meigymnophallus minutus in Cardium edule. J Invertebr Pathol. 1974;23:92–100.
Weiss M, Leese F. Widely distributed and regionally isolated! Drivers of genetic structure in Gammarus fossarum in a human-impacted landscape. BMC Evol Biol. 2016;16:153.
Westram AM, Jokela J, Baumgartner C, Keller I. Spatial distribution of cryptic species diversity in European freshwater amphipods (Gammarus fossarum) as revealed by pyrosequencing. PLoS ONE. 2011;6:e23879.
We thank Maria Texieira, Tomasz Rewicz and Tomasz Mamos for the help with animal sampling and for molecular works.
We acknowledge French national research agency (ANR) (Grant # ANR-15-CE32-0006) for paying AQ salary, for contribution in designing the study, molecular analyses, statistical analyses, writing the manuscript and paying publication fees; the Polish Ministry of Science and Education (Grants # N303 579439 and N304 350139) for funding animal sampling, analyzing the results and writing the manuscript; as well as ‘Polonium’ exchange program (PPN/BIL/2018/1/00138), for funding travels between countries for designing the study, analyzing the results and writing the manuscript.
Ethics approval and consent to participate
All the applicable international, national, and/or institutional guidelines for the care and use of animals were followed and were in agreement with recommendations from the "Comité d’Ethique et de l’Expérimentation Animale (C2EA Dijon Grand Campus) from Dijon University, France. No experiments on alive animals were performed for this study.
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Details of the Dictyocoela spp. infections in the 88 populations investigated over the geographic range of Gammarus balcanicus.
Individual-wise data for Dictyocoela spp. infections from this study and found in Genbank (NCBI), mainly for fresh and brackish waters Amphipoda species present in Europe and Lake Baikal.
Worksheet 1, Matrices of genetic distances used for the PACo analyses illustrated on Fig. 6. Worksheet 2, Details of the all the PACo analyses.
Maximum-Likelihood phylogenetic reconstruction based on small ribosomal subunit (SSU) rDNA for the microsporidian genus Dictyocoela (detailed version of Fig. 3). Maximum-Likelihood phylogenetic reconstruction based on small ribosomal subunit rDNA for the microsporidian genus Dictyocoela infecting amphipods. Four taxa including infections in G. balcanicus were ascribed a color i.e. D. duebenum (grey), D. muelleri (red), D. roeselum (green) and D. berillonum sl (blue). While the three later clades strictly reflect recent reassessment of the genus taxonomy by Bacela-Spychalska et al. (2018), the former one putatively extends this taxon i.e. D. duebenum sl (see text and Additional files 2, 5). Sequences used for species formal description by Bacela-Spychalska et al.  are indicated with a star (*). Haplogroups from the present study are in bold. Dictyocoela sequences from Genbank include the accession number and the host species abbreviated name(s) (see Additional file 2). Values at nodes are bootstrap values > 50%.
Maximum-Likelihood phylogenetic reconstruction based on small ribosomal subunit rDNA, as well as ITS and LSU when available, for taxon sub-sampling of Additional file 4, aiming at providing evidence of support of some key clades. Four taxa including infections in Gammarus balcanicus were ascribed a color i.e. Dictyocoela duebenum (grey), D. muelleri (red), D. roeselum (green) and D. berillonum sl (blue). Values at nodes are bootstrap values. Specimens representative of diversity and divergence within each clade were used. Sequences used for species formal description by Bacela-Spychalska et al.  are indicated with a star (*).
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Quiles, A., Wattier, R.A., Bacela-Spychalska, K. et al. Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity. BMC Evol Biol 20, 149 (2020). https://0-doi-org.brum.beds.ac.uk/10.1186/s12862-020-01719-z
- Host-parasite relationships
- SSU rDNA
- Gammarus balcanicus