Primer and adaptor sequences | Target | Product size | Thermocycling conditions |
---|---|---|---|
Forward Primer V418SNextFor: | 18 S rRNA V4 | 378 bp | 5 min at 95 °C, 30 × (30 s at 95 °C, 30 s at 55.2 °C, 30 s at 72 °C), 5 min at 72 °C. |
 5’-[TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG]  CCAGCASCYGCGGTAATTCC-3’ | |||
Reverse primer V418SNextRev: | |||
 5’-[GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG]  ACTTTCGTTCTTGATYRATGA-3’ |