Table 1 Non-synonymous mutations associated with Nicaraguan DENV-2 clades
Gene | Position | NI | NI-3 | NI-3 A | NI-3B | NI-3B.1 | NI-3B.2 | NI-3B.3 | Changes in hydropathy | Changes in charge | Changes in polarity |
|---|---|---|---|---|---|---|---|---|---|---|---|
(1999- 2002) | (2013- 2019) | (2013- 2015 | (2015- 2019) | (2015- 2019) | (2016- 2019) | (2018- 2019) | |||||
prM | 31 | V (GTT) | V (GTT) | V (GTT) | V (GTT) | I (ATT) | I (ATT) | I (ATT) | None | None | None |
E | 118 | M (ATG) | M (ATG) | K (AAG) | M (ATG) | M (ATG) | M (ATG) | M (ATG) | Hydrophobic to Hydrophilic | Uncharged to Positively charged | Nonpolar to Polar |
E | 200 | T (ACA) | T (ACA) | I (ATA) | T (ACA) | T (ACA) | T (ACA) | T (ACA) | Neutral to Hydrophobic | None | Polar to Nonpolar |
NS1 | 172 | R (AGA) | R (AGA) | R (AGA) | K (AAA) | K (AAA) | K (AAA) | K (AAA) | None | None | None |
NS1 | 247 | F (TTT) | F (TTT) | F (TTT) | L (TTA) | L (TTA) | L (TTA) | L (TTA) | None | None | None |
NS2A | 136 | I (ATA) | V (GTA) | V (GTA) | V (GTG) | V (GTG) | V (GTG) | V (GTG) | None | None | None |
NS2A | 139 | N (AAT) | N (AAT) | K (AAA) | N (AAT) | N (AAT) | N (AAT) | N (AAT) | None | Uncharged to Positively charged | None |
NS2B | 39 | T (ACC) | T (ACC) | T (ACC) | T (ACC) | T (ACC) | I (ATC) | I (ATC) | Neutral to Hydrophobic | None | Polar to Nonpolar |
NS4A | 10 | M (ATG) | M (ATG) | M (ATG) | M (ATG) | I (ATA) | I (ATA) | I (ATA) | Neutral to Hydrophobic | None | Polar to Nonpolar |
NS4A | 78 | I (ATA) | I (ATA) | I (ATA) | I (ATA) | V (GTA) | V (GTA) | V (GTA) | None | None | None |
NS4B | 48 | V (GTC) | V (GTC) | V (GTC) | L (CTC) | L (CTC) | L (CTC) | L (CTC) | None | None | None |
NS4B | 156 | E (GAA) | D (GAC) | D (GAC) | D (GAC) | D (GAC) | D (GAC) | D (GAC) | None | None | None |
NS5 | 271 | I (ATA) | I (ATA) | I (ATA) | V (GTA) | V (GTA) | V (GTA) | V (GTA) | None | None | None |
NS5 | 521 | D (GAC) | E (GAA) | E (GAA) | E (GAA) | E (GAA) | E (GAA) | E (GAA) | None | None | None |
NS5 | 641 | I (ATC, ATT*) | I (ATC) | I (ATC) | I (ATC) | I (ATC) | I (ATC) | T (ACC) | Hydrophobic to Neutral | None | Nonpolar to Polar |