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Table 2 Microsatellite markers for Pocillopora corals used in this study. The published size range refers to the cited publication, whereas the adapted size range refers to the range extended to the markers in this study.

From: Population genetic differentiation of the ubiquitous brooding coral Pocillopora acuta along Phuket Island reefs in the Andaman Sea, Thailand

Name

Motif

Published Size Range [bp]

Adapted Size Range [bp]

Dye

Tm

Multiplex Panel (MP) / Individual PCR

Reference

Pd4

(AAAC)5

130–190

130–190

6-FAM

60°C

MP1

[76]

Pd3-009a

(CAA)7 88bp insert (GAG)6

339–358

293–342

6-FAM

65°C

Individual PCR; MP1 post-PCR

[122]

Pd11

(CA)7 T (AC)13

120–180

120–180

VIC

59°C

MP1

[76]

Pd13

(TCTT)5

130–194

130–194

NED

61°C

MP1

[76]

Pd3-EF65a

(GTT)5 (TGC)11

259–281

194–215

PET

60°C

Individual PCR; MP1 post-PCR

[109]

Pd3-008

(CTG)7

153–162

151–176

6-FAM

53°C

MP2

[122]

Pd2-001a

(CA)11

158–173

196–208

VIC

64°C

Individual PCR; MP2 post-PCR

[122]

Pd2-006

(CA)8

181–199

182–202

NED

60°C

MP2

[122]

Pd3-004

(ATG)8

193–201

147–168

6-FAM

62°C

MP3

[122]

PV2b

(GA)20

130–196

124–196

VIC

66°C

MP3

[120]

Poc40a

(CAA)

289–316

293–320

6-FAM

56°C

Individual PCR; MP4 post-PCR

[121]

PV7b

(GT)5(CT)2GT(CT)3

215–233

214–232

VIC

65°C

MP4

[120]

Pd3-005a

(TGA)9

162–187

164–224

NED

60°C

MP4

[122]

  1. aMicrosatellite loci excluded from analysis due to low fraction of amplification
  2. bMicrosatellite loci excluded due to null alleles possibly resulting from technical errors