Table 1 Primers used for nested PCR
Application | Primer (Forward/Reverse) | Sequence (5′–3′ direction) | Amplification round (nested PCR) | Target (genus) | References |
|---|---|---|---|---|---|
General (all bird species) | HaemNF1 HaemNR3 | CATATATTAAGAGAANTATGGAG TAGAAAGATAAGAAATACCATTC | Nest1 | Haemoproteus, Plasmodium, Leucocytozoon | [61] |
HaemF HaemR2 | ATGGTGCTTTCGATATATGCATG GCATTATCTGGATGTGATAATGGT | Nest 2 | Haemoproteus, Plasmodium | ||
HaemFL HaemR2L | ATGGTGTTTTAGATACTTACATT CATTATCTGGATGAGATAATGGIG | Nest 2 | Leucocytozoon | [16] | |
Specific (raptors) | Plas1 HaemNR3 | GAGAATTATGGAGTGGATGGTG ATAGAAAGATAAGAAATACCATTC | Nest 1 | Haemoproteus, Plasmodium, Leucocytozoon | [47] |
3760F HaemJR4 | GAGTGGATGGTGTTTTAGAT GAAATACCATTCTGGAACAATATG | Nest 2 | Haemoproteus, Plasmodium, Leucocytozoon |