Fig. 2From: Lacking catalase, a protistan parasite draws on its photosynthetic ancestry to complete an antioxidant repertoire with ascorbate peroxidasePAGE analysis of APX activity and protein during the purification of APX activity. Peak APX fractions were separated by nondenaturing 10% PAGE. a Gel stained with Coomassie blue. b APX activity was visualized by a nitroblue tetrazolium stain. Lanes 1: Peak APX fraction from DEAE sephacel (30 μg protein). Lanes 2: Peak APX fraction from phenyl Sepharose, Lanes 3: The fraction following the APX peak activity from phenyl Sepharose. c SDS-PAGE analysis of peak APX fraction. d Sequences of tryptic peptides identified by Edman degradation from the 32 kDa band in (c)Back to article page