Fig. 7From: Double maternal-effect: duplicated nucleoplasmin 2 genes, npm2a and npm2b, with essential but distinct functions are shared by fish and tetrapodsEffect of nucleoplasmin (npm) 2a and npm2b deficiencies on zebrafish embryogenesis. Representative images demonstrating development of fertilized eggs from crosses between control (a-d), npm2a (e-l), or npm2b (m-p) females and wildtype (WT) males from 2 to 24 h post-fertilization (hpf). In the control eggs, the embryos were at 64-cell (a), oblong (b), germ ring (c), and 24-somite (d) stages according to Kimmel et al. [23]. Eggs from npm2a mutant females were non-developing with a normal morphology (e-h) or with an abnormal morphology (i-l). Eggs from npm2b mutant females had a normal morphology albeit were developing abnormally (e-h). (a, e, i, m) = images taken at 2 hpf; (b, f, j, n) = images taken at 4 hpf; (c, g, k, o) = images taken at 6 hpf; (d, h, l, p) = images taken at 24 hpf. Scale bars denote 400 μm. Red dotted lines define the diameter of the embryo. Arrows demonstrate a partially cellularized blastodisc that was sitting atop an enlarged yolk syncytial layerBack to article page