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Table 1 Primers used for RT-PCR on eleven Takifugu rubripes tissues.

From: Early vertebrate chromosome duplications and the evolution of the neuropeptide Y receptor gene regions

Gene Forward primer Reverse primer Size of fragment
Y2 5'-ACTCTCATCTACACGCTGTACGG-3' 5'-CATCAGCAACCATCTTGGTGGTCTT-3' 484
Y4 5'-TCATGGACCATTGGGTGTTTGGCTC-3' 5'-GCCATGCGCTGGCACTCTGGA-3' 601
Y7 5'-CACTCTGGTTTACACTCTGCTGGAT-3' 5'-TCGTTGCGAGTGGATGGGCTGA-3' 453
Y8a 5'-ACCGCTGGGTGCTGGGAGAG-3' 5'-CGTCGTTTCAGGCGAAGGAAGAT-3' 481
Y8b 5'-GACCGCTGGATCCTGGGCGAT-3' 5'-TGTTTCTCTTCTGGGCGCCGT-3' 460 (267)
Actin 5'-AACTGGGAYGACATGGAGAA-3' 5'-TTGAAGGTCTCAAACATGAT-3' 130