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Table 4 Time constraints used for divergence time estimation

From: Evolutionary history of Otophysi (Teleostei), a major clade of the modern freshwater fishes: Pangaean origin and Mesozoic radiation

Node

Constraints

Calibration information

A

U 472

The minimum age for the basal split of bony fish based on the earliest known acanthodian remains from Late Ordovician [128]

 

L 419

The †Psarolepis fossil (sarcopterygians) [129] from Ludlow (Silurian) [100]

B

U 419

The minimum age for the Sarcopterygii/Actinopterygii split

 

L 392

The †Moythomasia fossil (actinopteran) from the Givetian/Eifelian boundary [100]

C

U 392

The minimum age for the Polypteriformes/Actinopteri split

 

L 345

The †Cosmoptychius fossil (neopterygian or actinopteran) from Tournasian [100]

D

L 130

The †Protopsephurus fossil (Polyodontidae) from Hauterivian (Cretaceous) [100]

E

L 284

The †Brachydegma fossil (stem amiids) from Artinskian (Permian) [100]

F

L 136

The †Yanbiania fossil (Hiodontidae) from the Lower Cretaceous [100]

G

L 112

The †Laeliichthys fossil (Osteoglossidae) from the Aptian (Cretaceous) [130]

H

L 151

The †Anaethalion, †Elopsomolos, and †Eoprotelops fossil (Elopomorpha) from Kimmeridgian (Jurassic) [100]

I

L 94

The †Lebonichthys (Albulidae) fossil from the Cenomanian (Cretaceous) [130]

J

L 49

The Conger (Congridae) and Anguilla (Anguillidae) fossils from the Ypresian (Tertiary) [130]

K

L 146

The †Tischlingerichthys fossil (Ostariophysi) from Tithonian (Jurassic) [100]

L

L 56

The †Knightia fossil (Clupeidae) from the Thanetian (Paleogene) [130]

M

L 49

The †Parabarbus fossil (Cyprinidae) from the Ypresian (Paleogene) [130]

N

L 8

Gymnotiform fossil from the Late Miocene [131]

O

L 98

The Santanichthys (Characiformes) fossil from Albian (Cretaceous) [94]

P

L 74

The ariid fossil from Campanian (Cretaceous) [132]

Q

L 74

The †Esteseox foxi fossil (Esociformes) from the Campanian (Cretaceous) [133]

R

L 94

The †Berycopsis fossil (Polymixiidae) from the Cenomanian (Cretaceous) [130]

S

L 98

The tetraodontiform fossil from the Cenomanian [134]

T

L 32

The estimated divergence time between Takifugu and Tetraodon [135]

U

U 95 L 85

The upper and lower bounds of separation between Madagascar and Indian [104]

V

U 145 L 112

The upper and lower bounds of separation between Indo-Madagascar landmass and Gondwanaland [104]

W

U 120 L 100

The upper and lower bounds of separation between African and South American landmasses [104]

  1. Maximum (U) and minimum (L) time constraints in million years ago (Ma) at nodes depicted in Additional file 1