Widely used, short 16S rRNA mitochondrial gene fragments yield poor and erratic results in phylogenetic estimation and species delimitation of amphibians

Table 2 Results of the ASAP species delimitation analysis

Number of species	ASAP score	p-val (rank)	W (rank)	Threshold dist
Full
31	2	2.00e−05 (2)	4.56e−04 (2)	0.077418
36	4.5	7.56e−03 (4)	3.24e−04 (5)	0.04772
59	8.5	1.42e−01 (7)	2.44e−04 (10)	0.014968
32	11.5	1.04e−02 (5)	2.12e−04 (18)	0.069101
26	13.5	1.00e−05 (1)	1.84e−04 (26)	0.094478
Medium
37	2	7.51e−04 (3)	6.35e−04 (1)	0.039249
36	2.5	1.10e−04 (2)	4.14e−04 (3)	0.06046
39	9.5	5.08e−02 (8)	2.24e−04 (11)	0.034695
27	10	1.00e−05 (1)	1.16e−04 (19)	0.086903
39	10	6.60e−03 (7)	1.63e−04 (13)	0.032463
Short
37	9	8.57e−04 (2)	2.26e−04 (16)	0.033333
51	9.5	3.73e−01 (14)	3.99e−04 (5)	0.013921
29	11	1.87e−03 (3)	2.18e−04 (19)	0.072876
29	13.5	1.06e−01 (9)	2.23e−04 (18)	0.073809
50	14	5.77e−01 (19)	3.38e−04 (9)	0.014268

A lower ASAP score indicates a better species partition. The ASAP score is the average of ranks from the p-val and W parameters combined. p-val: probability of panmixia; W: relative gap width metric. See Puillandre et al. [42] for more details

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