Fig. 6
From: The evolution of the metazoan Toll receptor family and its expression during protostome development
Expression of TLRs during the development of the brachiopod T. transversa. Whole-mount in situ hybridization (WMISH) of TLRs in T. transversa embryos and larvae. Above the WMISH plates, there are schematic representations of each developmental stage analyzed. These representations are not to scale. The name of each gene is indicated in the rectangles on the left. All panels show dorso-ventral views and anterior to the top. Squares in the top-right of each plate indicate whether the expression was detected (yellow) or not (blue) in the stage-specific transcriptome analysis. Ectoderm, mesoderm and endoderm is indicated with blue, red and yellow arrowheads, respectively. The red and yellow arrowhead indicates endomesoderm. The ring-shape (indicated with white asterisks) staining present in the late larvae is background staining (Additional file 10: Fig. S4), probably related with the spicule formation described by Stricker and Reed [126,127,128]. Scale bar indicates 50 μm. al: apical lobe; bp: blastopore; cs: chaetal sacs; em: endomesoderm; me: mesoderm; ml: mantle lobe; pl: pedicle lobe