Skip to main content

Cospeciation of coronavirus and paramyxovirus with their bat hosts in the same geographical areas

Abstract

Background

Bat-borne viruses are relatively host specific. We hypothesize that this host specificity is due to coevolution of the viruses with their hosts. To test this hypothesis, we investigated the coevolution of coronavirus and paramyxovirus with their bat hosts. Published nucleotide sequences of the RNA-dependent RNA polymerase (RdRp) gene of 60 coronavirus strains identified from 37 bat species, the RNA polymerase large (L) gene of 36 paramyxovirus strains from 29 bat species, and the cytochrome B (cytB) gene of 35 bat species were analyzed for coevolution signals. Each coevolution signal detected was tested and verified by global-fit cophylogenic analysis using software ParaFit, PACo, and eMPRess.

Results

Significant coevolution signals were detected in coronaviruses and paramyxoviruses and their bat hosts, and closely related bat hosts were found to carry closely related viruses.

Conclusions

Our results suggest that paramyxovirus and coronavirus coevolve with their hosts.

Background

Bats are reservoirs of many zoonotic viruses, such as members of Filoviridae (e.g., Ebola and Marburg viruses), Paramyxoviridae (e.g., Hendra and Nipah viruses), and Coronaviridae (e.g., severe acute respiratory syndrome coronavirus, Middle East respiratory syndrome coronavirus, severe acute respiratory syndrome coronavirus-2) [1, 2]. Bats live in a wide variety of environments with various feeding habits. They are flying mammals and are effective vehicles for spreading viruses [3].

Coronaviruses are taxonomically placed in the subfamily Coronavirinae under the family Coronaviridae (International Committee on Taxonomy of Viruses). Bats coronaviruses have been shown to be responsible for the outbreaks of severe acute respiratory syndrome (SARS) in 2002–2003, Middle East respiratory syndrome (MERS) in 2012 [4, 5], and probably the current COVID-19 [2]. They cause serious respiratory and instetinal symptoms with substantial mortality rates [6].

Some paramyxoviruses such as Hendra virus (HeV) and Nipah virus (NiV) are highly pathogenic zoonoses. Both viruses belong to the genus Henipavirus of the family Paramyxoviridae (International Committee on Taxonomy of Viruses) and have been detected in flying fox bats (Pteropus spp.) [7, 8]. Henipavirus causes severe symptoms associated with high mortality rates in humans and livestocks. HeV was first detected in Queensland, Australia in 1994, causing acute respiratory disease and febrile illness in horses and humans who have close contact with sick horses [9]. NiV was first detected in Malaysia in 1999 during the outbreak of encephalitis and respiratory illness in pig farmers. In the past few years, sporadic outbreaks of HeV and NiV have occurred in Oceania and Southeast Asia [10,11,12].

Both coronaviruses and paramyxoviruses have a certain degree of host specificity. Host–parasite specificity has also been observed in malaria parasites [13], bat flies [14], and bacteria [15]. We hypothesized that the host specificity of coronaviruses and paramyxoviruses is a result of co-speciation with their hosts. To test this hypothesis, we compared the nucleotide sequences of the cytochrome B (cytB) gene of 61 bat species with those of the RNA dependent RNA polymerase (RdRp) gene of 60 coronavirus strains and the RNA polymerase large (L) gene of 36 paramyxovirus strains.

Results

Phylogenetic relationship of bat coronaviruses with their bat hosts

Phylogenetic analyses revealed that the bat coronavirus RaTG13/CN/MN996532 from Rhinolophus affinis found in Yunnan Province, China is on the same branch of the phylogenetic tree as the 18 human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains examined, suggesting that it is a relative of SARS-CoV-2 (Fig. 1). The following 18 bat coronavirus strains are clustered on the same branch of the phylogenetic tree as the six human severe acute respiratory syndrome coronavirus (SARS-CoV) analyzed, suggesting that they are phylogenetic relatives: Rf1/CHN/DQ412042, JTMC15/CHN/KU182964, JL2012/CHN/KJ473811, 16BO133/ROK/KY938558, HeB2013/CHN/KJ473812, SX2013/CHN/KJ473813, Shaanxi2011/CHN/JX993987, Rm1/CHN/DQ412043, HuB2013/CHN/KJ473814, HKU3-1/CHN/DQ022305, Yunnan2011/CHN/JX993988, As6526/CHN/KY417142, YN2013/CHN/KJ473816, Rs3367/CHN/KC881006, GX2013/CHN/KJ473815, Anlong-103/CHN/KY770858, Rp3/CHN/DQ071615, and LYRa11/CHN/KF569996. The strain BM48-31/BGR/GU190215 from Rhinolophus blasii living in Bulgaria is found to be distantly related to the six human SARS-CoVs as it is located alone on a branch of the phylogenetic tree (Fig. 1). The following 13 bat coronavirus strains are clustered on the same branch of phylogenetic tree as the six human Middle East respiratory syndrome coronavirus (MERS-CoV) strains examined, suggesting that they are evolutionarily close to each other: JPDB144/CHN/KU182965, HKU4-1/CHN/EF065505, GX2012/CHN/KJ473822, HKU5-1/CHN/EF065509, GD2013/CHN/KJ473820, PDF-2180/UGA/KX574227, 5038/RSA/MF593268, PML-PHE1/RSA/KC869678, SC2013/CHN/KJ473821, HKU25/CHN/KX442565, NL13845/CHN/MG021451, 206645-40/ITA/MG596802, and 206645-63/ITA/MG596803 (Fig. 1).

Fig. 1
figure 1

Phylogenetic analysis of the 2734-bp RNA-dependent RNA polymerase (RdRp) gene of coronaviruses from humans and various species of bats

Coevolution of bat coronaviruses with their bat hosts

Analyses of all bat cytB gene sequences and all bat coronavirus RdRp gene sequences as a whole by the Global test function of software ParaFit and PACo showed evidence of coevolution between bat coronaviruses and their bat hosts (ParaFitGlobal = 390.8896, P = 0.001; m2 global value = 57.136, P ≤ 0.001). When each individual sequence was analyzed by the Individual host–parasite (H–P) link test function of ParaFit, 51 of the 60 bat coronavirus strains were found to have a significant coevolution relationship (link) with their bat hosts with a ParaFit1 or ParaFit2 P value ≤ 0.05.

Bat coronavirus strains examined in this study are divided into alpha and beta groups (Fig. 1). In the alphacoronavirus group, 1B/CHN/EU420137, AH2011/CHN/KJ473795, 1A/CHN/EU420138, HKU7-1/CHN/DQ249226, and HKU8/CHN/EU420139 are related and are all derived from Miniopterus bats. Strains SAX2011/CHN/KJ473806, Anlong-57/CHN/KY770851, and Anlong-43/CHN/KY770850 are also related and are all from Myotis bats. Similarly, KW2E-F151/GHA/KT253269 and AT1A-F1/GHA/KT253272 are related and are all from Hipposideros bats. These observations suggest host specificity of these bat coroanviruses. The following coroanviruses are closely related but are from different species of bats of the same family (Vespertilionidae): Lushi-212/CHN/KF294373 (from Murina leucogaster), 16715 23/VIE/MH687934 (from Scotophilus kuhlii), HKU6-1/CHN/DQ249224 (from Myotis ricketti), and CDPHE15/USA/KF430219 (from Myotis lucifugus) (Fig. 2). This result suggests that some coronaviruses have a less stringent host specificity than others.

Fig. 2
figure 2

Tanglegram of cophylogenetic relationship between bat hosts and coronaviruses. Black lines denote significant coevolution links between coronaviruses and their hosts (ParaFit tests P ≤ 0.05), and gray lines denote non-significant links. Different groups of coronaviruses and bat species with significant coevolution links are marked with boxes in different colors. Information on host geographical distribution was derived from Simmons (2005)

In this study, bat coroanviruses that are related to human SARS-CoVs are referred to as SARSr-CoVs, and those that are related to human MERS-CoVs are referred to as MERSr-CoVs. These viruses are clustered on the betacoronavirus branch of the phylogenetic tree (Fig. 1). Most bat SARSr-CoVs are found in members of the Rhinolophidae bat family, and most bat MERSr-CoVs are derived from members of the Vespertilionidae bat family. Strains CMR66/CMR/MG693170, HKU9-1/CHN/EF065513, and GCCDC1-356/CHN/KU762338 with 78.03–96.24% RdRp gene sequence identity are clustered together on the same branch of the phylogenetic tree and are all from members of the Pteropodidae bat family (Fig. 2).

Strains 206645-40/IT/MG596802 and 206645-63/IT/MG596803 are related with 99.46% identity in RdRp gene sequence and are found in Hypsugo savii and Pipistrelle kuhli, respectively, in Italy (Fig. 2). The SARSr-CoV 16BO133/ROK/KY938558 found in Rhinolophus ferrumequinum in South Korea is evolutionarily close to JL2012/KJ473811 (99.71% RdRp sequence identity) and JTMC15/KU182964 (99.68% RdRp sequence identity) found in the same species (Rhinolophus ferrumequinum) of bats in Jilin Province, China [16] (Fig. 2). The strain As6526/CHN/KY417142 is found in Aselliscus stoliczkanus (family Hipposideridae) that are evolutionarily close to Rhinolophidae bats (Fig. 2). The strain Yunnan2011/CHN/JX993988 found in Yunnan Province, China is from Chaerephon plicatus (family Molossidae) bats that are distantly related to Rhinolophidae bats (Fig. 2).

Coevolution of bat paramyxoviruses with their bat hosts

Analyses of all bat cytB gene sequences and all paramyxovirus RNA polymerase large (L) gene sequences as a whole by the Global test function of ParaFit and PACo showed evidence of coevolution between bat paramyxoviruses and their bat hosts (ParaFitGlobal = 874.11, P = 0.049; m2 global value = 15.49537, P = 0.015). When each individual sequence was analyzed by the Individual H–P link test function of ParaFit, 7 of the 36 bat paramyxovirus strains were found to have a significant host–parasite coevolution relationship (link) with a ParaFit1 or Parafit2 P value ≤ 0.05.

In this study, we classified unidentified bat paramyxoviruses into four groups PG1–PG4 according to their host speficity. PG1 paramyxovirus strains GB59-59/GHA/HQ660162, GB09670/GAB/HQ660156, GB59-30/GHA/HQ660161, GH19-140/GHA/HQ660153, GD2012/CHN/KJ64165, and GB09682/GAB/HQ660157 are closely related and are all derived from Hipposideros bats (family: Hipposideridae) (Fig. 3). The following PG2 paramyxovirus strains are form Pteropodidae bats and are closely related: RCA-P18/RCA/HQ660152, CD273/DRC/HQ660122, GB1386/GAB/HQ660137, GB1237/GAB/HQ660140, and GH6/GHA/FJ971938 (Fig. 3). KCR245H/CRC/JF828297, BR21/BRA/HQ660187, BR310/BRA/HQ660194, BR310/BRA/HQ660194, and BR190/BRA/HQ660190 that belong to paramyxovirus group 3 (PG3) are closely related (Fig. 3). The host of KCR245H/CRC/JF828297 is Pteronotus parnellii bat (family: Mormoopidae), and the hosts of the other four strains are bats of the Pteropodidae family, including Desmodus rotundus, Carollia perspicillata, Carollia brevicauda, and Glossophaga soricina (Fig. 3). Seven closely related strains, including GH36/GHA/FJ609192, 3-320/BGR/HQ660163, N78-14/GER/HQ660166, 6-43/BGR/HQ660164, NMS09-48/GER/HQ660165, Md-LN2012/CHN/KJ641656, and NM98-46/GER/HQ660170 (paramyxovirus group 4, PG4), are found in members of the Vespertilionidae bat family (Fig. 3). Identified bat paramyxoviruses including Teviot virus (TeV), Tioman virus (TiV), and Menangle virus (MENV) are members of the genus Pararubulavirus; their bat hosts are members of the Pteropodidae family. The bat hosts of Henipavirus, NiV, and HeV also belong to the Pteropodidae family (Fig. 3).

Fig. 3
figure 3

Tanglegram of cophylogenetic relationships between bat hosts and paramyxoviruses. Black lines denote significant coevolution links between paramyxoviruses and their hosts (ParaFit tests P ≤ 0.05), and gray lines denote non-significant links. Different groups of paramyxoviruses and bat species with significant coevolution links are marked with boxes in different colors. Information on host geographical distribution was derived from Simmons (2005)

The bat hosts of PG1 paramyxoviruses are distributed mainly in Africa and Asia, and those of PG2 paramyxoviruses are mostly living in Africa. PG3 paramyxoviruses are mostly found in bats in South and North America. The bat hosts of PG4 paramyxoviruses are distributed in Asia, Africa, and Europe. The bat hosts of Pararubulavirus and Hennipahvirus are found in areas from Asia to Oceania (Fig. 3).

Event-based cophylogeny

To confirm the coevolution of coronaviruses and paramyxoviruses with their bat hosts, analyses with the software eMPRess were performed. Results of such analyses refuted the null hypothesis that the host and virus trees and tip associations are formed due to chance at 0.01 level in both host–coronavirus and host–paramyxovirus relationships (P-value = 0.0099). Therefore, we, concluded that these host–virus pairs have coevolved (Fig. 4).

Fig. 4
figure 4

P-value histogram of (A) host–coronavirus and (B) host–paramyxovirus relationships. The optimal reconciliation cost of the coevolution trees is indicated with a red line, and the optimal cost of the same trees constructed with tip associations permuted at random is shown in blue columns

Disccusion

In this study, we investigated the coevolution relationship between bats and their viral parasites: coronaviruses and paramyxoviruses. These two groups of viruses were chosen because they have been shown to be zoonotic [17]. The sequences of the RNA-dependent RNA polymerase (RdRp) gene of 60 bat coronavirus strains, the RNA polymerase large (L) gene of 36 paramyxovirus strains, and the cytochrome B (cytB) gene of 61 bat species were used to build phylogenetic trees. Cophylo analyses were then performed to determine the relationship between bat genetic trees and those of coronaviruses and paramyxoviruses. Event-based eMPRess test and ParaFit and PACo Global and ParaFit Individual H–P tests were also performed. In eMPRess and Global ParaFit and PACo tests, both groups of the viruses were found to have a significant coevolution relationship with their bat hosts. In ParaFit Individual H–P test, 51 (85%) of the 60 coronavirus strains and 7 (19%) of the 36 paramyxovirus strains had a significant coevolution relationship with their bat hosts.

The construction of cophylogenetic trees revealed that closely related bat coronaviruses are found in closely related bat species (Fig. 2). One example of such observation is that closely related coronavirus strains 1B/CHN/EU420137, AH2011/CHN/KJ473795, 1A/CHN/EU420138, and HKU8/CHN/EU420139 are found in Miniopterus pusillus, Miniopterus fuliginosus, and Miniopterus magnater that are very close to each other. Another example is the coevolution relationship between closely related bats including Tylonycteris pachypus, Hypsugo savii, Vespertilio sinensis, Neoromicia capensis, and Ia io and the following coronavirus strains: GX2012/CHN/KJ473822, 206645-40/ITA/MG596802, SC2013/CHN/KJ473821, NL13845/CHN/MG021451, 5038/RSA/MF593268, and PML-PHE1/RSA/KC869678. Similar coevolution relationships are found in closely related bat species Rhinolophus ferrumequinum, Rhinolophus blasii, Rhinolophus pusillus, Rhinolophus macrotis, Rhinolophus sinicus, Rhinolophus pearsonii, and Rhinolophus affinis and the following coronavirus strains: RF1/CHN/DQ412042, JTMC15/CHN/KU182964, JL2012/CHN/KJ473811, 16BO133/ROK/KY938558, HeB2013/CHN/KJ473812, SX2013/CHN/KJ473813, Shaanxi2011/CHN/JX993987, HuB2013/CHK/KJ473814, HKU3-1/CHN/DQ022305, YN2013/CHK/KJ473816, Rs3367/CHN/KC881006, GX2013/CHN/KJ473815, Anlong-103/CHN/KY770858, Rp3/CHN/DQ071615, LYRa11/CHN/KF569996, and RaTG13/CHN/MN996532 (Fig. 2). For paramyxoviruses, closely related bat species Neoromicia nanus, Myotis alcathoe, Myotis myotis, Myotis capaccinii, Myotis daubentoniid, and Myotis bechsteinii are found to carry the following closely related strains: GH36/GHA/FJ609192, 3-320/BGR/HQ660163, N78-14/GER/HQ660166, 6-43/BGR/HQ660164, NMS09-48/GER/HQ660165, LN2012/CHN/KJ641656, and NM98-46/GER/HQ660170 (Fig. 3).

Results of ParaFit Individual H–P test showed that 85% (51/60) of coronavirus strains but only 19% (7/36) of paramyxovirus strains have a significant coevolution relationship with their bat hosts. Since significant coevolution was found in both groups of the viruses by eMPRess and ParaFit and PACo Global tests, this low positive individual H–P link rate in paramyxoviruses may be due to small sample size.

We postulate that that evolutionary relationship and close habitat of bat species contribute to inter-species transmission of viruses. One observation supporting this hypothesis is that coronavirus strains 206645-40/IT/MG596802 (bat host: Hypsugo savii) and 206645-63/IT/MG596803 (bat host: Pipistrellus kuhlii) share 99.46% nucleotide sequence identity in their RdRp gene and are found in bats living in the same geographical area, Italy. In addition, coronavirus strains 16BO133/ROK/KY938558, JL2012/KJ473811, and JTMC15/KU182964 are highly related with > 99.6% nucleotide sequence identity in the RdRp gene and are found in bats distributing in areas near each other, including Jilin Province, China (for isolates JL2012/KJ473811 and JTMC15/KU182964) and South Korea (for isolate 16BO133/ROK/KY938558) that is close to China.

Most bat SARSr-CoVs are from Rhinolophus bats, but strains As6526/CHN/KY417142 and Yunnan2011/CHN/JX993988 are found in Aselliscus stoliczkanu and Chaerephon plicatus, respectively. Although these two bat species are phylogenetically far apart, they live in the same geographical area, Yunnan Province, China. This observation suggests that distantly related bats in the same geographical location may harbor the same viruses, leading to inter-species transmission of the viruses.

Horseshoe bats are widely distributed in Europe, Asia, Australia, and Africa [18,19,20,21] and are potential reservoirs of epidemic coronaviruses [22]. The strain BM48-31/BGR/GU190215 was found in Rhinolophus blasii and clustered with other SARSr-CoVs in the coronavirus coevolution tree (Fig. 2). Its relationship with human and civet SARS-CoVs is farther than that of other SARSr-CoVs (Fig. 1), suggesting that they diverged from the same ancestor. Results of previous studies suggest that Rhinolophus bats are the natural hosts of human SARS-CoVs [23,24,25]. Many bat SARSr-CoVs are detected in Rhinolophus sinicus and Rhinolophus ferrumequinum that distributed in Asia, Africa, and Europe. However, SARSr-CoVs that are highly related to human SARS-CoVs have not been found in Rhinolophus ferrumequinum that live in Africa and Europe, probably due to geopgraphical isolation from SARSr-CoVs that are mainly found in Yunnan Province, China [26].

For paramyxoviruses, Pteropid bats have been shown to be the natural reservoir of Henipavirus [27, 28] and are speculated to be responsible for the outbreaks in Malaysia, Australia, Singapore, Philippine, India, and Bangladesh during the period of 1995–2015 [29,30,31,32,33,34,35]. Several species of Pteropid bats, including Pteropus alecto, Pteropus conspicillatus, Pteropus giganteus, and Pteropus vampyrus, have been found to carry Henipaviurs. These bats live in Southeast Asia and Oceania [36], where Henipavirus pandemic occurred.

As Rhinolophus sinicus bats are found only in China, Nepal, Vietnam, and North India [36] and SARSr-CoVs are mainly found in Yunnan Province, China, SARS-CoV outbreaks have not occurred in other places such as Europe, Africa, Oceania, and America. SARS-CoV-2 is responsible for the COVID-19 pandemic (International Committee on Taxonomy of Viruses). In this study, the strain RaTG13/CHN/MN996532 is found to be very close to SARS-CoV-2 with > 97% nucleotide sequence identity in the RdRp gene and 96% identity at the whole genome level. There are approximately 1100 bases that are different between the genomes of RaTG13/MN996532 and various strains of SARS-CoV-2 suggesting that RaTG13/MN996532 requires at least one intermediate host to transmit to humans [2]. As the host of RaTG13/MN996532 is Rhinolophus affinis residing in Yunnan Province, China, it has been speculated that SARS-CoV-2 is derived from Rhinolophus bats roosting in areas near Yunnan Province, China, such as Southwest China, Myanmar, Laos, Vietnam, and other Southeast Asian countries [37].

Divergence of bats can be traced back to tens of million years ago [38, 39]. It has been estimated that coronaviruses diverged tens of thousand years ago [40]. This difference may be due to the fact that the genome of coronaviruses is RNA that is more prone to mutations than DNA. It has also been estimated that coronaviruses have been infecting birds and bats for tens of million years, thus providing the opportunity for coevolution with their hosts [41].

Conclusion

We have found evidence suggesting that both coronavirus and paramyxovirus coevolve with their bat hosts. Understanding the coevolutionary patterns of these viruses with their hosts will allow a better prediction of transmission between bats and humans.

Methods

Phylogenetic analysis

The database of Bat-associated Viruses (DBatVir, http://www.mgc.ac.cn/DBatVir/) [42] contains information on various bat-associated viruses, including genome size, lengths of identified genes, date and place (city and country) of isolation, names of the viruses, and GenBank accession numbers of nucleotide sequences of the entire genome or individual genes. With the “browse by virus” function, 60 coronavirus and 36 paramyxovirus strains with all aforementioned information available were found. As this database does not contain actual sequences, nucleotide sequences of selected viral strains were downloaded from the GenBank. These sequences included those of the RNA dependent RNA polymerase (RdRp) gene (2734 bp) of the 60 coronaviruse strains and the RNA polymerase large (L) gene (559 bp) of the 36 paramyxovirus strains. The 60 coronaviruses were derived from 37 different bat species, and the 36 paramyxoviruses were derived from 29 different bat species. Among them, 5 bat species including Myotis daubentoniid, Eidolon helvum, Hipposideros abae, Hipposideros ruber, and Hipposideros pomona were found to harbor both coronavirus and paramyxovirus. Altogether, 61 different species of bats were identified to be the hosts of the coronavirus and paramyxovirus strains examined in this study. As the cytochrome B (cytB) gene is one of the most conserved gene in bats, its sequence was used for coevolution analyses. The cytB sequences of 59 of the 61 bat species were downloaded from the GenBank. Since the cytB gene sequences of Miniopterus pusillus and Tylonycteris robustula, that were the hosts of coronavirus strains 1B/CHN/EU420137 and HKU33/CHN/MK720944, respectively, were not available, they were determined in this study. These two bat species were captured from Menghai, Yunnan and Kau O Bat Cave, Macau, respectively. Anal swabs of Miniopterus pusillus and a small portion of the patagiums of Tylonycteris robustula were obtained. The captured bats were released back to their roosts after sampling. DNA was isolated from these samples and used as the template for amplication by polymerase chain reaction (PCR) of a portion (1140 bp) of the cytB gene with primers L14727ag (5′-ATGATATGAAAAACCATCGTTG) and H15915ag (5′-TTTCCNTTTCTGGTTTACAAGAC) [43]. PCR conditions were as follows: 94 °C for 3 min, followed by 20 cycles of 94 °C for 20 s, 46 °C to 52 °C (+ 0.3 °C/cycle) for 30 s, and 72 °C for 90 s and 30 cycles of 94 °C for 20 s, 60 °C for 30 s, 72 °C for 90 s and then maintained at 72 °C for 10 min. The PCR products were sequenced, and the sequences thus obtained have been deposited in the Genbank with accessing numbers MN366287 and MN366288.

To analyze the nucleotide sequences, they were aligned with Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo) [44]. The outgroup sequences included in sequence alignments were those of coronavirus Turkey COV/NC_01080, paramyxovirus Sosugavirus/KF774436, and bat Megaderma lyra/DQ888678. Maximum-likelihood phylogenetic trees were constructed using 1000 bootstraps with the raxmlGUI program [45] using the substitution model GTR + I + G, which compares likelihood scores calculated with the jmodeltest 2.1.7 software [46].

Comparison of host and virus phylogenies

To determine the degree of congruence between bat and virus topologies on phylogenetic trees and identify individual associations contributing to the cophylogenetic relationship the software ParaFit was used [47]. ParaFit tests the null hypothesis that the evolutions of a clade of hosts and a clade of parasites are independent. ParaFit has two types of tests: a global test of coevolution and a test on each host–parasite (H–P) link. The matrices of patristic distances used in global-fit analysis were calculated from the maximum likelihood trees of host and virus phylogenies using the “cophenetic” function of the software package ape in R 3.6.0 [48, 49]. ParaFit analyses were also performed in R with 999 permutations for both global and individual H–P link tests. Each individual host–virus link was considered as significant when its ParaFit 1 or Parafit 2 P-value was ≤ 0.05 [15].

To obtain comparable global goodness-of-fit statistics with Parafit global values, the software package Procrustean Approach to Cophylogeny (PACo) [50] in R was used in conjunction with packages ape and vegan [51]. PACo determines the dependence of one phylogeny upon the other and produces a Procrustes superimposition plot for a graphical assessment of the fit of the parasite phylogeny onto the host phylogeny and a goodness-of-fit statistic. As ParaFit values may be variable, virus matrix in PACo was rotated and scaled to fit the host matrix in order to evaluate the dependence of parasite phylogeny upon host phylogeny. A goodness-of-fit test based on 1000 randomizations was used to assess the significance of such dependence. The associated squared residuals were used to assess the significance of coevolution of each host–virus link [52]. Cophylogenetic trees were generated using the “cophylo” function of the R package phytools [53].

Even-based cophylogeny

The event-based program eMPRess [54] was used to determine whether the pairs of coronavirus, paramyxovirus, and their hosts coevolve with each other. eMPRess is a tool for reconciling pairs of phylogenetic trees based on the Duplication-Transfer-Loss (DTL) model [55], which is performed using a maximum parsimony formulation to determine the associated cost of each coevolution event. eMPRess computes and displays the distances between every pair of maximum parsimony reconciliation (MPR). The distance between two MPRs is the number of events in one MPR. The maximum likelihood (ML) trees of hosts and viruses were used as inputs, and the analysis was conducted using the following eMPRess parameters: duplication cost = 1, transfer cost = 2, and loss cost = 1. The optimal reconciliation cost for each dataset was compared with that of the same tree with tip associations permuted at random.

Availability of data and materials

The nucleotide sequences of the cytB gene of Miniopterus pusillus and Tylonycteris robustula bats are available at GenBank with accession numbers MN366287 and MN366288.

Abbreviations

RdRp:

RNA-dependent RNA polymerase

L:

RNA polymerase large gene

cytB :

Cytochrome B

HeV:

Hendra virus

NiV:

Nipah virus

TeV:

Teviot virus

TiV:

Tioman virus

MENV:

Menangle virus

H–P:

Host–parasite

SARS-CoV-2:

Severe acute respiratory syndrome coronavirus 2

SARS-CoV:

Severe acute respiratory syndrome coronavirus

MERS-CoV:

Middle East respiratory syndrome coronavirus

SARSr-CoV:

Bat coronavirus related to human SARS-CoV

MERSr-CoV:

Bat coronavirus related to human MERS-CoV

References

  1. Luis AD, Hayman DT, O’Shea TJ, Cryan PM, Gilbert AT, Pulliam JR, et al. A comparison of bats and rodents as reservoirs of zoonotic viruses: are bats special? Proc Biol Sci. 2013;280(1756):20122753.

    PubMed  PubMed Central  Google Scholar 

  2. Zhou P, Yang XL, Wang XG, Hu B, Zhang L, Zhang W, et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020;579:270–3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Serra-Cobo J, López-Roig M. Bats and emerging infections: an ecological and virological puzzle. Adv Exp Med Biol. 2017;972:35–48.

    Article  PubMed  Google Scholar 

  4. Zaki AM, van Boheemen S, Bestebroer TM, Osterhaus AD, Fouchier RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012;367(19):1814–20.

    Article  CAS  PubMed  Google Scholar 

  5. Drexler JF, Corman VM, Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antiviral Res. 2014;101:45–56.

    Article  CAS  PubMed  Google Scholar 

  6. Gralinski LE, Baric RS. Molecular pathology of emerging coronavirus infections. J Pathol. 2015;235(2):185–95.

    Article  CAS  PubMed  Google Scholar 

  7. Smith I, Broos A, de Jong C, Zeddeman A, Smith C, Smith G, et al. Identifying Hendra virus diversity in pteropid bats. PLoS ONE. 2011;6(9):e25275.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Anderson DE, Islam A, Crameri G, Todd S, Islam A, Khan SU, et al. Isolation and full-genome characterization of Nipah viruses from bats, Bangladesh. Emerg Infect Dis. 2019;25(1):166–70.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Selvey LA, Wells RM, McCormack JG, Ansford AJ, Murray K, Rogers RJ, et al. Infection of humans and horses by a newly described morbillivirus. Med J Aust. 1995;162(12):642–5.

    Article  CAS  PubMed  Google Scholar 

  10. Harit AK, Ichhpujani RL, Gupta S, Gill KS, Lal S, Ganguly NK, et al. Nipah/Hendra virus outbreak in Siliguri, West Bengal, India in 2001. Indian J Med Res. 2006;123(4):553–60.

    CAS  PubMed  Google Scholar 

  11. Mahalingam S, Herrero LJ, Playford EG, Spann K, Herring B, Rolph MS, et al. Hendra virus: an emerging paramyxovirus in Australia. Lancet Infect Dis. 2012;12(10):799–807.

    Article  PubMed  Google Scholar 

  12. Sharma V, Kaushik S, Kumar R, Yadav JP, Kaushik S. Emerging trends of Nipah virus: a review. Rev Med Virol. 2019;29(1):e2010.

    Article  PubMed  Google Scholar 

  13. Ricklefs RE, Fallon SM, Bermingham E. Evolutionary relationships, cospeciation, and host switching in avian malaria parasites. Syst Biol. 2004;53(1):111–9.

    Article  PubMed  Google Scholar 

  14. Nikon N, Sato M, Kondo N, Fukatsu T. Phylogenetic comparison between nycteribiid bat flies and their host bats. Med Entomol Zool. 2011;62(3):185–94.

    Article  Google Scholar 

  15. Lei BR, Olival KJ. Contrasting patterns in mammal–bacteria coevolution: bartonella and leptospira in bats and rodents. PLoS Negl Trop Dis. 2014;8(3):e2738.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Xu L, Zhang F, Yang W, Jiang T, Lu G, He B, et al. Detection and characterization of diverse alpha- and betacoronaviruses from bats in China. Virol Sin. 2016;31(1):69–77.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Rizzo F, Edenborough KM, Toffoli R, Culasso P, Zoppi S, Dondo A, et al. Coronavirus and paramyxovirus in bats from Northwest Italy. BMC Vet Res. 2017;13(1):396.

    Article  PubMed  PubMed Central  Google Scholar 

  18. DeBlase AF, Martin RL. Distributional notes on bats (Chiroptera: Rhinolophidae, Vespertilionidae) from Turkey. Mammalia. 1973;37:598–602.

    Article  Google Scholar 

  19. Krystufek B. Geographic variation in the greater horseshoe bat Rhinolophus ferrumequinum in south-eastern Europe. Acta Theriol. 1993;38:67–79.

    Article  Google Scholar 

  20. Maree S, Grant WS. Origins of horseshoe bats (Rhinolophus, Rhinolophidae) in southern Africa: evidence from allozyme variability. J Mamm Evol. 1997;4:195–215.

    Article  Google Scholar 

  21. Hand S, Kirsch AW. A southern origin for the Hipposieridae (Microchiroptera)? Evidence from the Australian fossil record. In: Kunz TH, Racey PA, editors. Bat biology and conservation. Smithsonian Institution Press: Washington; 1998. p. 72–90.

    Google Scholar 

  22. Lau SKP, Wong ACP, Zhang L, Luk HKH, Kwok JSL, Ahmed SS, et al. Novel bat alphacoronaviruses in southern China support Chinese horseshoe bats as an important reservoir for potential novel coronaviruses. Viruses. 2019;11(5):423.

    Article  CAS  PubMed Central  Google Scholar 

  23. Lau SK, Woo PC, Li KS, Huang Y, Tsoi HW, Wong BH, et al. Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats. Proc Natl Acad Sci USA. 2005;102(39):14040–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Ge XY, Li JL, Yang XL, Chmura AA, Zhu G, Epstein JH, et al. Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature. 2013;503(7477):535–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Yang XL, Hu B, Wang B, Wang MN, Zhang Q, Zhang W. Isolation and characterization of a novel bat coronavirus closely related to the direct progenitor of severe acute respiratory syndrome coronavirus. J Virol. 2015;90(6):3253.

    Article  PubMed  CAS  Google Scholar 

  26. Hu B, Zeng LP, Yang XL, Ge XY, Zhang W, Li B, et al. Discovery of a rich gene pool of bat SARSr-coronaviruses provides new insights into the origin of SARS coronavirus. PLoS Pathog. 2017;13(11):e1006698.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Young PL, Halpin K, Selleck PW, Field H, Gravel JL, Kelly MA, et al. Serologic Evidence for the presence in Pteropus bats of a paramyxovirus related to equine morbillivirus. Emerg Infect Dis. 1996;2(3):239–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Halpin K, Young PL, Field HE, Mackenzie JS. Isolation of Hendra virus from pteropid bats: a natural reservoir of Hendra virus. J Gen Virol. 2000;81(8):1927–32.

    Article  CAS  PubMed  Google Scholar 

  29. Murray K, Selleck P, Hooper P, Hyatt A, Gould A, Gleeson L, et al. A morbillivirus that caused fatal disease in horses and humans. Science. 1995;268(5207):94–7.

    Article  CAS  PubMed  Google Scholar 

  30. Chua KB, Goh KJ, Wong KT, Kamarulzaman A, Tan PS, Ksiazek TG, et al. Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia. Lancet. 1999;354(9186):1257–9.

    Article  CAS  PubMed  Google Scholar 

  31. Chua KB, Bellini WJ, Rota PA, Harcourt BH, Tamin A, Lam SK, et al. Nipah virus: a recently emergent deadly paramyxovirus. Science. 2000;288(5470):1432–5.

    Article  CAS  PubMed  Google Scholar 

  32. Hsu VP, Hossain MJ, Parashar UD, Ali MM, Ksiazek TG, Kuzmin I, et al. Nipah virus encephalitis re-emergence, Bangladesh. Emerg Infect Dis. 2004;10(12):2082–7.

    Article  PubMed  PubMed Central  Google Scholar 

  33. Chadha MS, Comer JA, Lowe L, Rota PA, Rollin PE, Bellini WJ, Ksiazek TG, et al. Nipah virus-associated encephalitis outbreak, Siliguri, India. Emerg Infect Dis. 2006;12(2):235–40.

    Article  PubMed  PubMed Central  Google Scholar 

  34. Arankalle VA, Bandyopadhyay BT, Ramdasi AY, Jadi R, Patil DR, Rahman M, et al. Genomic characterization of Nipah virus, West Bengal, India. Emerg Infect Dis. 2011;17(5):907–9.

    Article  PubMed  PubMed Central  Google Scholar 

  35. Ching PK, de los Reyes VC, Sucaldito MN, Tayag E, Columna-Vingno AB, Malbas FF Jr, et al. Outbreak of henipavirus infection, Philippines. Emerg Infect Dis. 2015;21(2):328–31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Simmons NB. Order Chiroptera. In: Wilson D, Reeder D, editors. Mammal species of the world: a taxonomic and geographic reference. 3rd ed. Johns Hopkins University Press: Baltimore; 2005. p. 312–529.

    Google Scholar 

  37. Latinne A, Hu B, Olival KJ, Zhu G, Zhang L, Li H, Daszak P, et al. Origin and cross-species transmission of bat coronaviruses in China. Nat Commun. 2020;11(1):4235.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Teeling EC, Springer MS, Madsen O, Bates P, O’brien SJ, Murphy WJ. A molecular phylogeny for bats illuminates biogeography and the fossil record. Science. 2005;307(5709):580–4.

    Article  CAS  PubMed  Google Scholar 

  39. Agnarsson I, Zambrana-Torrelio CM, Flores-Saldana NP, May-Collado LJ. A time-calibrated species-level phylogeny of bats (Chiroptera, Mammalia). PLoS Curr. 2011;3:1212.

    Article  Google Scholar 

  40. Woo PC, Lau SK, Lam CS, Lau CC, Tsang AK, Lau JH, et al. Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus. J Virol. 2012;86(7):3995–4008.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Wertheim JO, Chu DK, Peiris JS, Kosakovsky Pond SL, Poon LL. A case for the ancient origin of coronaviruses. J Virol. 2013;87(12):7039–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Chen L, Liu B, Yang J, Jin Q. DBatVir: the database of bat-associated viruses. Database (Oxford). 2014. https://0-doi-org.brum.beds.ac.uk/10.1093/database/bau021.

    Article  PubMed Central  Google Scholar 

  43. Guillén-Servent A, Francis CM. A new species of bat of the Hipposideros bicolor group (Chiroptera: Hipposideridae) from Central Laos, with evidence of convergent evolution with Sundaic taxa. Acta Chiropterol. 2006;8(1):39–61.

    Article  Google Scholar 

  44. Sievers F, Higgins DG. Clustal Omega, accurate alignment of very large numbers of sequences. Methods Mol Biol. 2014;1079:105–16.

    Article  CAS  PubMed  Google Scholar 

  45. Silvestro D, Michalak I. raxmlGUI: a graphical front-end for RAxML. Organ Diver Evol. 2012;12(4):335–7.

    Article  Google Scholar 

  46. Darriba D, Taboada GL, Doallo R, Posada D. Jmodeltest 2: more models, new heuristics and parallel computing. Nat Methods. 2012;9(8):772–772.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. Legendre P, Desdevises Y, Bazin E. A statistical test for host–parasite coevolution. Syst Biol. 2002;51(2):217–34.

    Article  PubMed  Google Scholar 

  48. Paradis E, Claude J, Strimmer K. Ape: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20(2):289–90.

    Article  CAS  PubMed  Google Scholar 

  49. R Core Team. R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. 2016. https://www.R-project.org/.

  50. Balbuena JA, Míguez-Lozano R, Blasco-Costa I. Paco: a novel procrustes application to cophylogenetic analysis. PLoS ONE. 2013;8(4):e61048.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O’Hara RB, et al. Vegan: Community Ecology Package. 2016. https://CRAN.R-project.org/package=vegan.

  52. Singh G, Grande FD, Divakar PK, Otte J, Crespo A, Schmitt I. Fungal–algal association patterns in lichen symbiosis linked to macroclimate. New Phytol. 2017;214(1):317–29.

    Article  PubMed  Google Scholar 

  53. Revell LJ. Phytools: an R package for phylogenetic comparative biology (and other things). Methods Ecol Evol. 2011;3(2):217–23.

    Article  Google Scholar 

  54. Santichaivekin S, Yang Q, Liu JY, Mawhorter R, Jiang J, Wesley T, et al. eMPRess: a systematic cophylogeny reconciliation tool. Bioinformatics. 2020;betaa978. https://0-doi-org.brum.beds.ac.uk/10.1093/bioinformatics/btaa978.

  55. Ma W, Smirnov D, Libeskind-Hadas R. DTL reconciliation repair. BMC Bioinformatics. 2017;18(Suppl 3):76.

Download references

Acknowledgements

We thank Professors Xinglou Yang, Yi-Hsuan Pan, and Ran Libeskind-Hadas for valuable advice in this study.

Funding

This work was supported by grants from the GDAS Special Project of Science and Technology Development (Grant No. 2018GDASCX-0107), the Guangdong Provincial Science and Technology Program (Grant No. 2018B030324001) and the Guangdong Province Natural Resources Services (Ecological Forestry Construction) Special Fund in 2021 (Grant No. 0721159001). The funding bodies had no role in the design of the study; collection, analysis, and interpretation of data; and writing of the manuscript.

Author information

Authors and Affiliations

Authors

Contributions

LZ and JL conceived the ideas and designed the experiments. LZ performed morphological identification of bats. JL performed experiments and analyzed data under the guidance of CZ. JL and LZ wrote the paper. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Libiao Zhang.

Ethics declarations

Ethics approval and consent to participate

This study was conducted in accordance with the Regulations for the Administration of Laboratory Animals (Decree No. 2, State Science and Technology Commission, People's Republic of China) and approved by the Guangdong Entomological Institute Animal Care Committee (No. GDEI-AE-2006001).

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liang, J., Zhu, C. & Zhang, L. Cospeciation of coronavirus and paramyxovirus with their bat hosts in the same geographical areas. BMC Ecol Evo 21, 148 (2021). https://0-doi-org.brum.beds.ac.uk/10.1186/s12862-021-01878-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/s12862-021-01878-7

Keywords