Fig. 6

vrille expression during the day and after RNA interference (RNAi). A Relative normalised expression of naturally cycling vrille at six time points, starting at ZT0 (Zeitgeber Time = sampling time every 4 h over a 24 h period), in LD 18:6 and 19 °C. Yellow represents the time of the day when the lights were on and dark grey when the lights were off. B Relative normalised expression levels of vrille in LacZ-injected and no-injection control females, and in females injected with dsRNA targeting on vrille 48 h after the injections at ZT16. Error bars represent bootstrapped 95% confidence intervals. Significance levels were obtained from linear model and only significant observations are shown: *P < 0.05, **P < 0.01 and ***P < 0.001