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Table 4 Statistical summary for testing of potential differences in the relative TIGER rate of evolution between different categories of positions

From: 18S rRNA variability maps reveal three highly divergent, conserved motifs within Rotifera

Data set

Between paired positions

Stems vs. loops

Non-hypervariable vs. hypervariable regions – counts of constant vs. variable

Non-hypervariable vs. hypervariable regions – rates

Among hypervariable as well as pooled non-hypervariable regions

Bdelloidea

Wilcoxon W = 2211.5; Z = 1.055; p = 0.292; nnon-zero,total = 88, 437

Mann–Whitney U = 1.604 × 105; Z = 2.969; p = 0.003; nstems,loops = 443, 776

χ2 = 105.70, df = 1; p = 8.60 × 10–25

Mann–Whitney U = 2.722 × 105; Z = 10.608; p = 2.74 × 10–26; nnon,hyper = 1022, 663

Kruskal–Wallis H = 75.3; p = 2.12 × 10–38

Monogononta

Wilcoxon W = 5142.0; Z = 1.881; p = 0.060; nnon-zero,total = 131, 458

Mann–Whitney U = 1.827 × 105; Z = 1.890; p = 0.059; nstems,loops = 461, 834

χ2 = 162.95, df = 1; p = 2.57 × 10–37

Mann–Whitney U = 2.613 × 105; Z = 13.217; p = 7.04 × 10–40; nnon,hyper = 1076, 683

Kruskal–Wallis H = 169.8; p = 6.92 × 10–59

Rotifera

Wilcoxon W = 11,856.0; Z = 1.0087; p = 0.313; nnon-zero,total = 209, 454

Mann–Whitney U = 1.866 × 105; Z = 1.913; p = 0.056; nstems,loops = 461, 859

χ2 = 130.18, df = 1; p = 3.74 × 10–30

Mann–Whitney U = 2.718 × 105; Z = 11.190; p = 4.54 × 10–29; nnon,hyper = 1079, 701

Kruskal–Wallis H = 174.3; p = 2.03 × 10–43

  1. Paired and unpaired positions were taken as proxies for stems and loops, respectively. Because paired rates were not significantly different from one another (column 2), each paired position was represented by only its single, average rate in column 3 to avoid problems with pseudoreplication. Only those positions that could definitely be assigned as being paired versus unpaired based on the structural map of the exemplar species (see Fig. 1) were included in the respective analyses