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Table 5 Microsatellite marker multiplex combinations, and associated information for primers used across populations of bearded vulture Gypaetus barbatus

From: Evidence of genetic structure in the wide-ranging bearded vulture (Gypaetus barbatus (Linnaeus, 1758))

Locus

Primer sequence (5′–3′)

Repeat motif

Ta (°C)

Label

Allele size range (bp)

Multiplex reaction

BV 9a

F: ATCTAGGGACATCGAGGAGC

(TA)6 (CA)11

58 °C

Hex

212

1

 

R: ACAGGGATGCAGGTAAGCC

     

BV14a

F: GGCAGTGTGGAGCCTACATC

(CA)16

58 °C

Fam

162–164

1

 

R: CTCCAGGGTCCTTGTTTGC

     

Gf11a4

F: GATCCCTTCCAACCGAAAAT

(CTCTT)17

58 °C

Hex

130–147

1

 

R: TGGTGACCAACGGAAGTGTG

     

BV2a

F: CAGCATGTTATTTTGGCTGC

(CA)11

60 °C

Hex

115–120

2

 

R:TTGCTAAACCGGTTAGAAGTTG

     

Gf8G

F: TGAGCAGGTGAGTCCAGAAG

(CT)8C(TC)2

60 °C

Fam

274

2

 

R: GCTCTCCTGTCATCTTGCAT

     

Gf3f3

F: GATCTTTCCCCTTCTGTG

(CT)10

60 °C

Tet

177–179

2

 

R: TTCGTGCAGTGATGCTGGTG

     

BV6a

F: AATCTGCATCCCAGTTCTGC

(CA)11

60 °C

Hex

106–119

3

 

R: CCGGAGACTCTCAGAACTTAAC

     

Gf3h3

F: GTAGAATAATTTGCTCCTGG

(CT)12

60 °C

Fam

137–141

3

 

R: GTGAAGGCACCTCATAGACA

     

Gf9C

F: GGTGGACATTACATACACTG

(TC)10 + (CT)9C (CA)5 T(AC)4

60 °C

Hex

262–268

3

 

R: CAAGGAATCTGGACTACTAA

     

BV5a

F: GTTCTGAGGGTAGAGGGACTG

(CA)17

58 °C

Tet

178

4

 

R: GCTGAGCAGCTTCAGAAAGTC

     

BV8a

F: TGGCATGCTGCTATGAGAAC

(CA)11

58°Cs

Fam

105

4

 

R: GTGCTTTGCATGCTTTTACTC

     

BV11a

F: TGTTTGCAAGCTGGAGACC

(CA)22

58 °C

Hex

160–162

4

 

R: AAAAGCCTTGGGGTAAGCAC

 

BV12a

F: TCAGGTTTTGACGACCTTCC

(CA)15

58 °C

Fam

256–269

4

 

R: GTGGTAACGGAGGAACAAGC

     

BV17a

F: TGATGTGCAGATGCGTGAC

(CA)11

62 °C

Hex

186

 
 

R: GGACTCTGATGAAGCCAAGC

     
  1. aSpecies-specific loci, the remaining are family specific
  2. bBV17 was run in isolation