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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: New insights into the genetic diversity of the stone crayfish: taxonomic and conservation implications

Fig. 3

Chronogram of 95% highest posterior density intervals (HPD) of divergence time estimates (in Ma) obtained with the mean values in brackets a using arthropod evolutionary rate [134, 135], b using decapod evolutionary rate [136], c using mid-points of a uniform distribution [137], d using geological calibration based on the connection of paleo-Tisza–paleo-Danube river systems e and g using geological calibration based on the uplift of the Dinaric Mountains [4, 61], f using geological event based on the separation of the Tisza–Dacia microplate from Dinarides [5]. Different colours denote the HPD of distinct lineages: dark blues—split of A. pallipes and A. torrentium; light blue—split of NCD (north and central Dinaric phylogroups = ZV, GK, LD, KOR, ŽPB) + APU from the BAN, SB and CSE phylogroups; purple—split of BAN from CSE + SB phylogroups; grey—split of CSE and SB phylogroups. In the upper right corner BEAST estimates of divergence times for A. torrentium based on the paleo-Danube–paleo-Tisza geological calibration is given; maximum clade credibility tree based on concatenated sequence. Horizontal node bars depict the 95% HPD intervals and are coloured according to posterior probability support (blue bars—posterior probabilities > 0.95; orange bars—posterior probabilities 0.50–0.95, green bars—posterior probabilities < 0.50. APU Apuseni, ZV Zeleni Vir, GK Gorski Kotar, LD Lika and Dalmatia, KOR Kordun, ŽPB Žumberak, Plitvice and Bjelolasica, BAN Banovina, SB southern Balkans, CSE central and southeastern Europe. Austropotamobius pallipes, Astacus astacus and Pontastacus leptodactylus were used as outgroups

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