Fig. 7

Gene expression in head EdU⁺ cells. a–e’ A 30-min EdU pulse (cyan) at different stages of neurogenesis was combined with FISH (red). f, g The number f and proportion g of EdU⁺ cells expressing a specific gene (colored boxes) in the brain were scored and plotted at different developmental stages. Anterior a, b, c, d, e and ventral a’, b′, c′, d’, e’ views of the anterior ectoderm are shown. Overlap of EdU⁺ cells with Ct-soxB1 (A, A’), Ct-ngn (B, B′), Ct-ash1c, c′, Ct-neuroDd, d’, and Ct-elav1e, e’ are shown. In panels A–E’, closed arrowheads indicate EdU⁺/gene⁺ cells while open arrowheads show EdU⁻/gene⁺ cells. Dashed orange lines in a’, b′, c′, d’, and e’, demarcate the basal boundary of the brain. In f, g, capital letters above the boxplots indicate statistical groups comparing cell counts or proportions at different stages based on ANOVA analysis. Boxplots with the same letter are not significantly different. Those with different letters are significantly different, with p < 0.05 after correction for multiple comparisons. Upper and lower bounds of the box plot indicate the 3rd and 1st quartiles while the middle line inside the boxplot indicates the median. The ends of the whiskers represent the 5th and 95th percentiles, black dots represent outliers (±3 S.D.). ant, anterior; vent, ventral. ST4mid: Stage 4 middle, ST4tt: Stage 4 telotroch, ST5: Stage 5, ST6: Stage 6. Scale bar: 50 μm