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Fig. 3 | BMC Evolutionary Biology

Fig. 3

From: The origins and developments of sulfation-prone tyrosine-rich and acidic N- and C-terminal extensions of class ll and lll small leucine-rich repeat proteins shed light on connective tissue evolution in vertebrates

Fig. 3

Alignments of class III SLRPs reveal the molecular evolution of their N-terminal regions. The upper panel in each subfigure is representative of sequences of the N-terminal regions of class III SLRPs from the class III SLRP MSAs (Additional file 1) starting from the first amino acid (position 1) after the signal peptide and ending at position 67 for mimecan and 65 for epiphycan and opticin. Lower panels are sequence logo representations of the N-terminal regions of each class III SLRP. Description of the residue colour coding can be found in the methods section. a: Mimecan representative sequences and sequence logo of tetrapod sequences (74 sequences). A large degree of variation in the length and amino acid composition of the N-terminal region of mimecan is evident from the number of gaps introduced in the MSA b: Epiphycan representative sequences and sequence logo of bony vertebrates sequences (77 sequences). Short extensions of the N-terminal region are found in ray-finned and cartilaginous fish at positions 9–14 and positions 19–24 and 32–39 in ghost shark epiphycan. c: Opticin representative sequences and sequence logo of bony vertebrates sequences (22 sequences). In lobe-finned fish and tetrapods the N-terminal region of opticin is extended with a sequence containing both basic and acidic residues (positions 8–19) compared to that in ray-finned fish. The representative sequences are from Homo sapiens (P20774; Q99645; Q9UBM4), Mus musculus (Q62000; P70186; Q920A0), Gallus gallus (Q9W6H0; Q6YEX8), Aptenodytes forsteri (A0A087RBX5), Chelonia mydas (M7CFR9; XP_007065189.1), Pelodiscus sinensis (K7F6Z9), Nanorana parkeri (XP_018407801.1), Xenopus tropicalis (Q6DK81; Q28HP1), Latimeria chalumnae (XP_005987115.1; XP_006009490.1; XP_006002321.1), Danio rerio (Q1LV51; Q5RI46; Q15JE7), Salmo salar (B5X6F3), Lepisosteus oculatus (W5NHX7), Scleropages formosus (A0A0P7UKK4), and Callorhinchus milii (V9L0I5; XP_007893502.1)

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