Fig. 6

Immunohistochemical staining of ovarian sections from maturing 2-year old salmon. a Buc1a restricted to granular ooplasm of immature oocytes with magnified section below showing immature oocytes positively stained for Buc1a (orange). b Buc2a in ooplasm of immature oocytes with magnified view of Buc2b positive oocytes (orange) below. c Vasa positive signals (orange) in immature oocytes with magnified view below. d Dazl signals (orange) in Bb and nucleoli of immature oocytes and granulosa cells of more mature follicles with magnified oocyte positively stained for Dazl. e Population of oocytes at different developmental stages showing Dnd signals (orange) in both immature and cortical alveolus stage oocytes. Magnified view of Dnd signals is shown below. Proteins are stained orange and nuclei are stained blue with DAPI. Ovaries used to prepare the sections were collected from 3 different females. Bb- Balbiani body, gc- granulosa cell, n- nucleolus. Negative (secondary antibody alone) and positive (collagen staining) controls are shown in Additional file 8: Figure S5