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Table 4 Detection of nucleotide composition at sites that are polymorphic in paired samples

From: Temporal variation and lack of host specificity among bacterial endosymbionts of Osedax bone worms (Polychaeta: Siboglinidae)

  

Nucl.

Dilution ratio

Mixture

Rep.

position

0:100

10:90

20:80

30:70

40:60

50:50

60:40

70:30

80:20

90:10

100:0

A x B

1

666

C

C

C

Y

Y

Y

Y

T

T

T

T

 

2

 

C

C

C

Y

Y

Y

Y

T

T

T

T

 

3

 

C

C

C

Y

Y

Y

Y

T

T

T

T

C x D

1

366

A

A

R

R

R

R

R

G

G

G

G

 

2

 

A

A

R

R

R

R

G

G

G

G

G

 

3

 

A

A

R

R

R

R

G

G

G

G

G

E x F

1

120

T

T

T

W

W

W

W

W

A

A

A

 

2

 

T

T

T

W

W

W

W

W

A

A

A

 

3

 

T

T

T

W

W

W

W

W

A

A

A

 

1

363

A

A

A

R

R

R

R

R

G

G

G

 

2

 

A

A

A

R

R

R

R

R

G

G

G

 

3

 

A

A

A

R

R

R

R

R

G

G

G

 

1

660

G

G

G

G

S

S

S

S

C

C

C

 

2

 

G

G

G

G

S

S

S

S

C

C

C

 

3

 

G

G

G

G

S

S

S

S

C

C

C

  1. Dilution series were created from mixtures of bacterial 16S amplicons obtained from the following Osedax samples: (A) T1048.115 clone E6; (B) T1048.115 clone D11; (C) T1071.13 clone D4; (D) T1071.13 clone C6; (E) worm T1071.35; (F) worm T1048.110. Each mixture was replicated three times. Mixtures detected by sequencing software are marked in boldface.