Figure 5
Statistical parsimony (95%) network for Campostoma ornatum RAG1 nuclear gene haplotypes. Statistical parsimony (95%) networks of 44 individuals of Campostoma ornatum sequenced for the nuclear gene RAG1. Networks were calculated (a) by treating heterozygous variable sites as unknown data and (b) using reconstructed haplotypes for each allele. Circle size reflects the frequency of each haplotype. Solid lines connecting each pair of haplotypes represent a single mutational event, regardless of their length. Small black rectangles represent missing or theoretical haplotypes. Haplotypes from Nazas and Aguanaval River basins are shadowed in green. River basin names are listed in parentheses.